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Herein, we investigated the function and mechanism of Evodiamine (EAE), the active ingredient from <jats:italic>Evodia rutaecarpa<\/jats:italic>, in chondrocytes and macrophages in vitro and in vivo. The cytotoxicity of EAE was determined using an MTT assay. And the anti\u2010inflammatory and anti\u2010extracellular matrix (ECM) degradation effects of EAE were investigated using qRT\u2010PCR, western blot (WB), immunofluorescence (IF). Inductively Coupled Plasma Atomic Emission Spectrometry (ICP\u2010AES), Fluo\u20104\u00a0AM, IF and AutoDock were used to elucidate the molecular mechanisms and signalling pathways of the reducing\u2010inflammatory properties of EAE on chondrocytes in vitro. Moreover, the effect of EAE on macrophage polarization was detected by IF and flow cytometry (FC). Ultimately, we explored the in vivo therapeutic efficacy of EAE in an anterior cruciate ligament transection (ACLT)\u2010induced OA model. The finding demonstrated that EAE blocked the phosphorylation of IKB\u03b1 and Ca<jats:sup>2+<\/jats:sup> influx, thereby curbing inflammation and ECM degradation. Additionally, EAE can prevent the polarization towards the M1 phenotype. Thus, our findings suggest that EAE has great potential as a therapeutic drug for the treatment of OA.<\/jats:p>","DOI":"10.1111\/bcpt.14096","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T06:35:26Z","timestamp":1729578926000},"page":"705-719","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The active ingredient of <i>Evodia rutaecarpa<\/i> reduces inflammation in knee osteoarthritis rats through blocking calcium influx and NF\u2010\u03baB pathway"],"prefix":"10.1111","volume":"135","author":[{"given":"Yan","family":"Gao","sequence":"first","affiliation":[{"name":"National Innovation and Attracting Talents \u201c111\u201d base, Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering Chongqing University  Chongqing 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