{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T13:04:47Z","timestamp":1789736687077,"version":"4.0.1"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82174051"],"award-info":[{"award-number":["82174051"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Ischemic stroke (IS) is one of the most sinister diseases and the second leading cause of death in the world. Eugenol (EUG) is a natural and biologically active component that can be extracted from various plants. Studies have found that EUG can alleviate middle cerebral artery occlusion and reperfusion (MCAO\/R) injury in mice, but the specific mechanism remains vague. Tripartite motif protein 59 (TRIM59) is a member of TRIM protein family, a group of E3 ubiquitin ligases. In this article, we conducted both in\u00a0vivo and in\u00a0vitro experiments to determine the effect of EUG on ischemia\u2013reperfusion injury and to explore the underlying mechanisms by manipulating the expression of TRIM59. Results showed that EUG alleviates acute injury and promotes functional repair of mouse IS by enhancing the phagocytosis of microglia through up\u2010regulating the TRIM59, activating the STAT3 pathway and promoting the expression of CD11b.<\/jats:p>","DOI":"10.1111\/bcpt.70058","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T06:59:12Z","timestamp":1748415552000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Eugenol Alleviates Cerebral Ischemia\u2013Reperfusion Injury in Mice by Promoting the Phagocytosis of Microglia via Up\u2010Regulating Tripartite Motif Protein 59"],"prefix":"10.1111","volume":"137","author":[{"given":"Mengtian","family":"Pan","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences and Clinical Pharmacy China Pharmaceutical University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Li","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences and Clinical Pharmacy China Pharmaceutical University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinjuan","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences and Clinical Pharmacy China Pharmaceutical University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lele\u00a0Zixin","family":"Yang","sequence":"additional","affiliation":[{"name":"State College The Pennsylvania State University, Eberly College of Science  Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0169-576X","authenticated-orcid":false,"given":"Weirong","family":"Fang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences and Clinical Pharmacy China Pharmaceutical University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568026621666210701141433"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2019.153158"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/2497354"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcme.2021.01.003"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcpt.13425"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021800"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000001052"},{"issue":"2","key":"e_1_2_9_9_1","first-page":"1272","article-title":"Neutrophil Membrane\u2010Derived Nanovesicles Alleviate Inflammation To Protect Mouse Brain Injury From Ischemic Stroke","volume":"13","author":"Dong X.","year":"2019","journal-title":"ACS Nano"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-0596-7288"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.00084"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-023-01337-5"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.790201"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2777-8"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.2147\/JIR.S469651"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.09.014"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9658-2_2"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032713-120240"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9658-2_3"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcpt.14109"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcpt.13944"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2022.0225"},{"issue":"5","key":"e_1_2_9_23_1","first-page":"1","article-title":"Middle Cerebral Artery Occlusion Model of Stroke in Rodents: A Step\u2010by\u2010Step Approach","volume":"8","author":"Shahjouei S.","year":"2016","journal-title":"Journal of Vascular and Interventional Neurology"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2020.12.009"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-015-0326-4"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.20.1.84"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.59.4.514"},{"key":"e_1_2_9_28_1","first-page":"8","article-title":"Motor Coordination and Balance in Rodents","volume":"8","author":"Carter R. 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