{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T02:56:40Z","timestamp":1786762600833,"version":"build-2736575974"},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Respiratory syncytial virus (RSV) is a leading etiological agent of pneumonia, particularly affecting infants under 2\u2009years and elderly populations, with characteristic clinical manifestations including fever, cough and wheezing. Although Feining mixture\u2014a traditional Chinese medicine formulation based on the classical Maxing Shigan Decoction from the \u2018Treatise on Febrile Diseases\u2019\u2014has shown clinical efficacy in ameliorating symptoms of viral pneumonias including RSV infection, its precise pharmacological mechanisms remain undefined. Through serum metabolomics and network pharmacology approaches, we systematically identified RSV\u2010associated metabolic disturbances and characterised Feining mixture's regulatory effects on these pathological alterations. Our investigations revealed that Feining mixture significantly attenuated pulmonary inflammation, as evidenced by reduced pro\u2010inflammatory cytokine levels in bronchoalveolar lavage fluid and improved histopathological features in RSV\u2010infected mice. Metabolomic profiling identified 49 differentially expressed metabolites and 14 perturbed metabolic pathways. Network pharmacology and molecular docking analyses predicted the involvement of TP53, AKT1 and STAT3 as core targets, suggesting modulation of PI3K\/AKT and TNF signalling pathways. These predictions were experimentally validated through qPCR and Wb analyses, which confirmed that Feining mixture's therapeutic effects are mediated through selective inhibition of the PI3K\/AKT1 signalling cascade. These findings provide novel mechanistic insights into Feining mixture's anti\u2010RSV activity, establishing its therapeutic potential through multi\u2010target modulation of critical inflammatory and metabolic pathways.<\/jats:p>","DOI":"10.1111\/bcpt.70114","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T07:42:10Z","timestamp":1758786130000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Metabolomics and Network Pharmacology Revealed the Mechanism of Feining Mixture Against Respiratory Syncytial Virus Pneumonia"],"prefix":"10.1111","volume":"137","author":[{"given":"Chengdou","family":"Xie","sequence":"first","affiliation":[{"name":"Nanjing University of Chinese Medicine  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Nanjing University of Chinese Medicine  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