{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T19:04:12Z","timestamp":1784401452408,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:00:00Z","timestamp":1775692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:00:00Z","timestamp":1775692800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to investigate the hepatotoxicity of camphor on zebrafish and its mechanism of toxicity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We calculated concentration\u2010response curves for zebrafish larvae treated with camphor at 72\u2010h postexposure (hpe) and observed its toxic effects on liver. Employing network pharmacology and molecular pairs to predict key targets of camphor\u2010induced hepatotoxicity, these critical targets were subsequently validated via qPCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>This study indicates that following 72\u2009hpe to camphor, the LC50 and LC10 values for zebrafish larvae were 336 and 300\u2009\u03bcg\/mL, respectively. Concentrations of 300\u2010 and 150\u2010\u03bcg\/mL camphor significantly reduced liver fluorescence intensity and markedly elevated ALT and AST levels in zebrafish. At 300\u2009\u03bcg\/mL, pathological alterations in liver tissue were observed, including vacuolation, disordered hepatocyte arrangement and loosening of tissue structure, indicating potential hepatic damage. Integrative analysis via network pharmacology, molecular docking and qPCR revealed that camphor induces hepatotoxicity through multiple targets and pathways, either directly or indirectly. Key targets included GSR, GCLC and NQO1, while major involved pathways encompassed drug metabolism\u2014other enzymes, chemical carcinogenesis\u2014receptor activation, biosynthesis of cofactors and glutathione metabolism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study has identified potential hepatotoxicity of camphor in zebrafish larvae, with its toxic mechanism involving multiple targets and pathways. The primary targets are likely GSR, GCLC and NQO1.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/bcpt.70222","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T04:36:16Z","timestamp":1775795776000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Network Pharmacology and Molecular Docking Reveals Mechanism of Camphor on Liver Toxicity in Zebrafish"],"prefix":"10.1111","volume":"138","author":[{"given":"Man","family":"Wei","sequence":"first","affiliation":[{"name":"School of Graduate Guangxi University of Chinese Medicine  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leihui","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Fangchenggang Hospital of Traditional Chinese Medicine  Fangchenggang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Gan","sequence":"additional","affiliation":[{"name":"School of Graduate Guangxi University of Chinese Medicine  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4158-0999","authenticated-orcid":false,"given":"Zhongshang","family":"Xia","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica Guangxi University of Chinese Medicine  Nanning China"},{"name":"University Engineering Research Center of Reutilization of Traditional Chinese Medicine Resources Guangxi University of Chinese Medicine  Nanning China"},{"name":"Guangxi Key Laboratory of TCM Formulas Theory and Transformation for Damp Diseases Guangxi University of Chinese Medicine  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengcai","family":"Du","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica Guangxi University of Chinese Medicine  Nanning China"},{"name":"University Engineering Research Center of Reutilization of Traditional Chinese Medicine Resources Guangxi University of Chinese Medicine  Nanning China"},{"name":"Guangxi Key Laboratory of TCM Formulas Theory and Transformation for Damp Diseases Guangxi University of Chinese Medicine  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0031\u20103955(16)35008\u20108"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40011\u2010022\u201001437\u20100"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.5348\/ijcri\u20102013\u201002\u2010267\u2010RA\u20101"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.18433\/J37C7K"},{"issue":"1","key":"e_1_2_11_6_1","article-title":"Biological Activity Prediction of an Ethno Medicinal Plant Cinnamomum camphora Through Bio\u2010Informatics","volume":"2008","author":"Chelliah D. 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