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We and others have recently shown that TRPA1 is also expressed in lung epithelial cells and that its expression is regulated by the cytokine environment. In the present study, we used next\u2010generation sequencing to analyse the role of TRPA1 and the effects of its inhibition on human A549 lung epithelial cell phenotype under inflammatory conditions. Two different TRPA1 inhibitors (HC\u2010030031 and A\u2010967079) were used and found to alter the expression of 968 genes: 576 genes were downregulated, and 392 upregulated. Ingenuity Pathway Analysis (IPA) predicted that TRPA1 inhibitors significantly suppress the activity of\n                    <jats:italic>pulmonary fibrosis idiopathic signalling<\/jats:italic>\n                    and\n                    <jats:italic>wound healing<\/jats:italic>\n                    pathways. In these pathways, altered genes included matrix metalloproteinases (MMPs), growth factors, collagens and transcription factors. The major profibrotic mediators MMP\u201012 and MMP\u20107 were among the most strongly inhibited genes by TRPA1 inhibitors. STRING network analysis suggests that TRPA1 regulates fibrosis\u2010related genes through FOS gene and AP\u20101 transcription factor. These findings together with previous data propose TRPA1 as a factor and therapeutic target in fibrotic lung diseases, which are associated with poor survival and critical need for novel disease\u2010modifying therapies.\n                  <\/jats:p>","DOI":"10.1111\/bcpt.70276","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T05:41:22Z","timestamp":1785994882000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["TRPA1 Regulates Fibrosis\u2010Associated Transcriptional Pathways in Human Lung Epithelial Cells"],"prefix":"10.1111","volume":"139","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1784-1936","authenticated-orcid":false,"given":"Leevi","family":"Halonen","sequence":"first","affiliation":[{"name":"The Immunopharmacology Research Group, Faculty of Medicine and Health Technology, Tampere University; and Tampere University Hospital, Wellbeing Services County of Pirkanmaa  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