{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:09:52Z","timestamp":1790154592023,"version":"4.0.1"},"reference-count":69,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Doxorubicin (DOX) and cyclophosphamide (CP) are widely used chemotherapeutic agents with known gonadotoxic effects. Since miRNAs play a key role in the transcriptional regulation of spermatogenesis, they are suggested as biomarkers for male infertility. This study aimed to investigate changes in\u00a0vitro the expression of proteins related to mitochondrial dynamics, apoptotic cell profiles and cell cycle distribution after treating mouse spermatogonia and spermatocytes with DOX and 4\u2010hydroperoxycyclophosphamide (4\u2010HC). Additionally, we also aimed to evaluate the correlation between mitochondrial function\u2013related miR\u201034 family expression and mitochondrial dynamics in both cells. Mitochondrial fusion, fission, mitophagy and apoptosis markers were analysed by immunocytochemistry and qPCR. miR\u201034 family expressions were assessed by qPCR. Apoptosis profiles and cell cycle distributions were determined using flow cytometry. We found that DOX and 4\u2010HC increased the percentage of cells in S and G2\/M phases and apoptosis levels. Mitochondrial fusion and mitophagy gene expressions decreased, whereas fission gene expression increased. Moreover, miR\u201034a expression rose, whereas miR\u201034b and miR\u201034c expressions significantly decreased after treatment. These miR\u201034 family expression changes correlated with mitochondrial dynamics\u2010related gene expressions, suggesting a potential role of the miR\u201034 family in mitochondrial dysfunction.<\/jats:p>","DOI":"10.1111\/bcpt.70304","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T10:22:39Z","timestamp":1789726959000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Doxorubicin and 4\u2010Hydroperoxycyclophosphamide Alter Mitochondrial Dynamics and miR\u201034 Expression in Spermatogonia and Spermatocytes In\u00a0Vitro"],"prefix":"10.1111","volume":"139","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6749-9665","authenticated-orcid":false,"given":"H\u00fclya","family":"\u00d6ztatl\u0131c\u0131","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Gaziantep University  Gaziantep 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