{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:10:07Z","timestamp":1790475007643,"version":"4.1.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,3,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Dermatophytes are common fungal pathogens causing mostly superficial infections in humans with a high prevalence worldwide. Traditional detection techniques are time-consuming and insensitive, whereas molecular detection methods have proved to be much more rapid and sensitive.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To develop a modular singleplex quantitative real-time polymerase chain reaction (qRT-PCR) assay for the detection of the most common dermatophytes in clinical specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The qRT-PCR assay is based on single-tube reactions with TaqMan probes. We validated the test with 311 clinical samples of human and animal origin submitted for routine diagnosis and compared the qRT-PCR results with microscopy and culture.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>qRT-PCR proved to be significantly more sensitive than microscopy and culture, with 21\u00b72% more positive samples. Among the 201 dermatophytes identified 152 were Trichophyton rubrum (75\u00b76%) and 34 were Trichophyton interdigitale (16\u00b79%). Only 15 samples were determined as less common dermatophytes (Microsporum canis, Epidermophyton floccosum, Trichophyton verrucosum and Arthroderma benhamiae). In the present study, pathogen identification was achieved for 95\u00b72% of all samples (including negatives) by applying only three detection tests (pan-dermatophyte, T. rubrum and T. interdigitale).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The qRT-PCR assay developed in this study allows the specific and sensitive detection of relevant dermatophytes at low cost in a short time.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bjd.14198","type":"journal-article","created":{"date-parts":[[2015,9,25]],"date-time":"2015-09-25T17:21:41Z","timestamp":1443201701000},"page":"602-609","source":"Crossref","is-referenced-by-count":54,"title":["Detection of common dermatophytes in clinical specimens using a simple quantitative real-time TaqMan polymerase chain reaction assay"],"prefix":"10.1093","volume":"174","author":[{"given":"T.","family":"Ohst","sequence":"first","affiliation":[{"name":"National Reference Laboratory for Dermatophytes Institute for Microbiology and Hygiene Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Rahel-Hirsch-Weg 3 10117 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Kupsch","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Dermatophytes Institute for Microbiology and Hygiene Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Rahel-Hirsch-Weg 3 10117 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Gr\u00e4ser","sequence":"additional","affiliation":[{"name":"National Reference Laboratory for Dermatophytes Institute for Microbiology and Hygiene Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Rahel-Hirsch-Weg 3 10117 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,3,1]]},"reference":[{"key":"2023033115534855000_","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1046\/j.0933-7407.2003.00933.x","article-title":"High prevalence of foot diseases in Europe: results of the Achilles Project","volume":"46","author":"Burzykowski","year":"2003","journal-title":"Mycoses"},{"key":"2023033115534855000_","doi-asserted-by":"crossref","first-page":"117","DOI":"10.3810\/pgm.2001.01.830","article-title":"Superficial fungal infection of the skin. 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