{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T13:56:47Z","timestamp":1784642207706,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,8,28]],"date-time":"2012-08-28T00:00:00Z","timestamp":1346112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>To determine the role of <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation in the progression of acute myeloid leukaemia (<jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content>), we analysed the methylation status of <jats:italic>ALOX12<\/jats:italic>,<jats:italic> GSTM1<\/jats:italic>,<jats:italic> HS3ST2<\/jats:italic> and <jats:italic>FZD9<\/jats:italic> in 127 <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content> patients. Aberrant methylation of <jats:italic>ALOX12<\/jats:italic> was associated with the subcategory <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content> with myelodysplasia\u2010related changes (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0\u00b70439) and specifically with megakaryocytic dysplasia (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0\u00b70003). An association between <jats:italic>HS3ST2<\/jats:italic> and AML patients with favourable cytogenetic risk was identified (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0\u00b70469). In univariate and multivariate analysis, methylation of <jats:italic>GSTM1<\/jats:italic> was associated with worse overall survival (<jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content>) and disease\u2010free survival (<jats:styled-content style=\"fixed-case\">DFS<\/jats:styled-content>), with hazard ratios of 2\u00b757 and 1\u00b786, respectively. Furthermore, the significance of methylation of <jats:italic>GSTM1<\/jats:italic> in predicting poor prognosis was maintained within the subcategories of <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content> not otherwise specified (<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content> with intermediate cytogenetic risk and normal karyotype <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content>. Finally, patients with both <jats:italic>GSTM1<\/jats:italic> and <jats:italic>ALOX12<\/jats:italic> methylated, demonstrated worse outcomes when all <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content> patients were assessed (<jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content>;<jats:italic> P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0\u00b7000411) as well as within <jats:styled-content style=\"fixed-case\">AML NOS<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">DFS<\/jats:styled-content>;<jats:italic> P\u00a0=\u00a0<\/jats:italic>0\u00b70023<jats:italic>), <\/jats:italic><jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content> with intermediate cytogenetic risk (<jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content>;<jats:italic> P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0\u00b70104) and normal karyotype <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content>;<jats:italic> P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0\u00b700636). This study implicates methylation of specific genes in the classification and prognostication of <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content> and suggests that the morphological feature of multilineage dysplasia may be a surrogate marker of gene methylation in at least a subset of <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content> cases.<\/jats:p>","DOI":"10.1111\/bjh.12029","type":"journal-article","created":{"date-parts":[[2012,8,28]],"date-time":"2012-08-28T05:46:08Z","timestamp":1346132768000},"page":"182-190","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["<scp>DNA<\/scp> methylation analysis of <i>ALOX12<\/i> and <i>GSTM1<\/i> in acute myeloid leukaemia identifies prognostically significant groups"],"prefix":"10.1111","volume":"159","author":[{"given":"Robert S.","family":"Ohgami","sequence":"first","affiliation":[{"name":"Department of Pathology Stanford University 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