{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T17:56:16Z","timestamp":1774374976196,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,8,30]],"date-time":"2012-08-30T00:00:00Z","timestamp":1346284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2012,11]]},"abstract":"Summary<\/jats:title>CD<\/jats:styled-content>1A<\/jats:styled-content> is a cell surface protein expressed on Langerhans cells and cortical thymocytes that could potentially be used as an immunotherapeutic target in Langerhans Cell Histiocytosis (LCH<\/jats:styled-content>), the cortical subtype of T<\/jats:styled-content>\u2010cell acute lymphocytic leukaemia (T<\/jats:styled-content>\u2010ALL<\/jats:styled-content>) and other CD<\/jats:styled-content>1A<\/jats:styled-content>\u2010positive tumours. The monoclonal antibody (mA<\/jats:styled-content>b) CR<\/jats:styled-content>2113 was selected from a panel of six fully human mA<\/jats:styled-content>bs isolated from a semi\u2010synthetic phage display library, based on specificity and avidity against cells expressing CD<\/jats:styled-content>1 antigen variants. CR<\/jats:styled-content>2113 recognized CD<\/jats:styled-content>1A<\/jats:styled-content> in T<\/jats:styled-content>\u2010ALL<\/jats:styled-content> cell lines and patient samples. Confocal microscopy revealed that the CR<\/jats:styled-content>2113\u2010CD<\/jats:styled-content>1A<\/jats:styled-content> complex was internalized at 37\u00b0C. Furthermore, while CR<\/jats:styled-content>2113 induced moderate complement\u2010dependent cytotoxicity (CDC<\/jats:styled-content>), potent antibody\u2010dependent cell cytotoxicity (ADCC<\/jats:styled-content>) activity was observed against CD<\/jats:styled-content>1A<\/jats:styled-content> expressing cell lines as well as T<\/jats:styled-content>\u2010ALL<\/jats:styled-content> cell lines and T<\/jats:styled-content>\u2010ALL<\/jats:styled-content> patient samples. In vivo<\/jats:italic> experiments showed that CR<\/jats:styled-content>2113 as a naked antibody has modest but specific anti\u2010tumour activity against CD<\/jats:styled-content>1A<\/jats:styled-content>\u2010expressing tumours. CR<\/jats:styled-content>2113 is a high\u2010affinity human anti\u2010CD<\/jats:styled-content>1A<\/jats:styled-content> mA<\/jats:styled-content>b with significant ADCC<\/jats:styled-content> activity. These properties make CR<\/jats:styled-content>2113 a candidate for clinical diagnostic imaging and therapeutic targeting of LCH<\/jats:styled-content> as well as potential use in other clinical applications.<\/jats:p>","DOI":"10.1111\/bjh.12033","type":"journal-article","created":{"date-parts":[[2012,8,30]],"date-time":"2012-08-30T14:19:14Z","timestamp":1346336354000},"page":"299-310","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Phage display generation of a novel human anti\u2010CD<\/scp>1A<\/scp> monoclonal antibody with potent cytolytic activity"],"prefix":"10.1111","volume":"159","author":[{"given":"Gitanjali I.","family":"Bechan","sequence":"first","affiliation":[{"name":"Department of Oncology Johns Hopkins Medical Institutions Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David W.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Oncology 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