{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T23:15:47Z","timestamp":1789859747249,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Extramedullary disease in patients with multiple myeloma is a rare event, occurring mostly in advanced disease or relapse. Outcome is poor and prognostic factors predicting the development of extramedullary disease have not been defined. We investigated cytogenetic alterations of myeloma cells in different extramedullary manifestations by adapting the fluorescence\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization (\n                    <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>\n                    ) technique in combination with cytoplasmic immunoglobulin staining to study the cytogenetics of plasma cell tumours on paraffin embedded material. Thirty six patients were investigated: 19 with extramedullary disease, 11 with skeletal extramedullary disease and six with solitary extramedullary plasmacytoma. The first two groups showed the following results: del(17p13) 32% vs. 27%, del(13q14) 35% vs. 27%,\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content>\n                    <\/jats:italic>\n                    \u2010overrepresentation 28% vs. 18% and t(4;14) 37% vs. 18%. We detected an overall higher incidence of del(17p13) in both groups compared to data from bone marrow samples of multiple myeloma reported to date (range 7\u201316%). The solitary extramedullary plasmacytomas presented overall less cytogenetic aberrations than the other groups. Most important, three patients with extramedullary disease and one with skeletal extramedullary disease presented different\n                    <jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>\n                    findings in the extramedullary tumour compared to their bone marrow plasma cells. del(17p13), occurring additional in three of four cases, seems a strong marker for extramedullary progression of myeloma.\n                  <\/jats:p>","DOI":"10.1111\/bjh.12223","type":"journal-article","created":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T10:40:25Z","timestamp":1359715225000},"page":"87-94","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":90,"title":["Cytogenetics of extramedullary manifestations in multiple myeloma"],"prefix":"10.1111","volume":"161","author":[{"given":"Lisa","family":"Billecke","sequence":"first","affiliation":[{"name":"Department of Oncology Haematology and Bone Marrow Transplantation with Section Pneumology Hubertus Wald Cancer Centre University Medical Centre Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva M.","family":"Murga Penas","sequence":"additional","affiliation":[{"name":"Department of Oncology Haematology and Bone Marrow Transplantation with Section Pneumology Hubertus Wald Cancer Centre University Medical Centre Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette M.","family":"May","sequence":"additional","affiliation":[{"name":"Department of Pathology Freiburg University Medical Centre Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Engelhardt","sequence":"additional","affiliation":[{"name":"Department of Haematology and Oncology Freiburg University Medical Centre Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnon","family":"Nagler","sequence":"additional","affiliation":[{"name":"Division of Haematology and Bone Marrow Transplantation Chaim Sheba Medical Centre Tel Hashomer Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merav","family":"Leiba","sequence":"additional","affiliation":[{"name":"Division of Haematology and Bone Marrow Transplantation Chaim Sheba Medical Centre Tel Hashomer Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ginette","family":"Schiby","sequence":"additional","affiliation":[{"name":"Division of Pathology Chaim Sheba Medical Centre Tel Hashomer Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolaus","family":"Kr\u00f6ger","sequence":"additional","affiliation":[{"name":"Department of Stem Cell Transplantation Hubertus Wald Cancer Centre University Medical Centre Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jozef","family":"Zustin","sequence":"additional","affiliation":[{"name":"Institute of Pathology University Medical Centre Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Marx","sequence":"additional","affiliation":[{"name":"Institute of Pathology University Medical Centre Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob","family":"Matschke","sequence":"additional","affiliation":[{"name":"Institute of Neuropathology University Medical Centre Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Tiemann","sequence":"additional","affiliation":[{"name":"Institute of Haematopathology Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eray","family":"Goekkurt","sequence":"additional","affiliation":[{"name":"Department of Oncology Haematology and Stem Cell Transplantation University Hospital Aachen Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans\u2010Heinrich","family":"Heidtmann","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine St. Joseph\u2010Hospital Bremerhaven Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eik","family":"Vettorazzi","sequence":"additional","affiliation":[{"name":"Department of Medical Biometry and Epidemiology University Medical Centre Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Dierlamm","sequence":"additional","affiliation":[{"name":"Department of Oncology Haematology and Bone Marrow Transplantation with Section Pneumology Hubertus Wald Cancer Centre University Medical Centre Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carsten","family":"Bokemeyer","sequence":"additional","affiliation":[{"name":"Department of Oncology Haematology and Bone Marrow Transplantation with Section Pneumology Hubertus Wald Cancer Centre University Medical Centre Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgia","family":"Schilling","sequence":"additional","affiliation":[{"name":"Department of Oncology Haematology and Bone Marrow Transplantation with Section Pneumology Hubertus Wald Cancer Centre University Medical Centre Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471249688"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.6.2185"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-08-040410"},{"key":"e_1_2_8_5_1","first-page":"2031","article-title":"Similar incidences of TP53 deletions in extramedullary organ infiltrations, soft tissues, and osteolyses of patients with multiple myeloma","volume":"32","author":"Billecke L.","year":"2012","journal-title":"Anticancer Research"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.12005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.34.9290"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2004.05199.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.011064"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2011.29"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(01)80838-8"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197509)36:3<842::AID-CNCR2820360303>3.0.CO;2-U"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-10-3017"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-2876"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2009.174"},{"key":"e_1_2_8_16_1","first-page":"47","article-title":"Clinical outcome of extramedullary plasmacytoma","volume":"85","author":"Galieni P.","year":"2000","journal-title":"Haematologica"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.26447"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.1.3"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2009.01.036"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1680"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-12-4435"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc746"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2009.01399.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705982"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1991.tb08620.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.22061"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2010.06.011"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-012-1414-5"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2010.11.002"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.88"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.22080"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2005.00531.x"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04355.x"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdp329"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-21706-2"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1704713"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbjh.12223","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.12223","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T14:54:54Z","timestamp":1697640894000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.12223"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1111\/bjh.12223"],"URL":"https:\/\/doi.org\/10.1111\/bjh.12223","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2]]},"assertion":[{"value":"2012-10-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-12-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}