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In this study, we carried out genome\u2010wide micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>) microarray analysis on diagnostic bone marrow samples to determine mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression profiles associated with poor outcome in <jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content>. A reduced expression of <jats:italic><jats:styled-content style=\"fixed-case\">MIR<\/jats:styled-content>335<\/jats:italic> was identified as the most significant mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> abnormality associated with poor outcome. It is well known that glucocorticoid (<jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>) resistance is one of the major reasons contributing to poor outcome. We show that exogenous expression of <jats:italic><jats:styled-content style=\"fixed-case\">MIR<\/jats:styled-content>335<\/jats:italic> in <jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content> cells increases sensitization to prednisolone\u2010mediated apoptosis. Moreover, we demonstrate that <jats:italic><jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>1<\/jats:italic> is a novel target of <jats:italic><jats:styled-content style=\"fixed-case\">MIR<\/jats:styled-content>335<\/jats:italic>, and that <jats:styled-content style=\"fixed-case\">MEK<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content> inhibitor treatment enhanced prednisolone\u2010induced cell death through the activation of <jats:styled-content style=\"fixed-case\">BIM<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">BCL<\/jats:styled-content>2L11). These results provide a possible underlying molecular mechanism to explain the association between reduced <jats:italic><jats:styled-content style=\"fixed-case\">MIR<\/jats:styled-content>335<\/jats:italic> with poor clinical outcome, and suggest that approaches to re\u2010introduce <jats:italic><jats:styled-content style=\"fixed-case\">MIR<\/jats:styled-content>335<\/jats:italic> expression or override <jats:italic><jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>1<\/jats:italic> activity may offer promising therapeutic strategies in the treatment of <jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/bjh.12489","type":"journal-article","created":{"date-parts":[[2013,7,25]],"date-time":"2013-07-25T17:29:26Z","timestamp":1374773366000},"page":"93-103","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Deregulated <i><scp>MIR<\/scp>335<\/i> that targets <i><scp>MAPK<\/scp>1<\/i> is implicated in poor outcome of paediatric acute lymphoblastic leukaemia"],"prefix":"10.1111","volume":"163","author":[{"given":"Junli","family":"Yan","sequence":"first","affiliation":[{"name":"Cancer Science Institute of Singapore National University of Singapore Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Yong Loo Lin School of Medicine National University of Singapore Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaofeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Cancer Science Institute of Singapore National University of Singapore Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jim L.\u2010S.","family":"Tay","sequence":"additional","affiliation":[{"name":"Department of Medicine Yong Loo Lin School of Medicine National University of Singapore Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baohong","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Haematology\u2010Oncology National University Cancer Institute of Singapore National University Health System Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chonglei","family":"Bi","sequence":"additional","affiliation":[{"name":"Cancer Science Institute of Singapore National University of Singapore Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grace S.","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Yong Loo Lin School of Medicine National University of Singapore Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenhua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Yong Loo Lin School of Medicine National University of Singapore Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joy","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Medicine Yong Loo Lin School of Medicine National University of Singapore Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae\u2010Hoon","family":"Chung","sequence":"additional","affiliation":[{"name":"Cancer Science Institute of Singapore National University of Singapore Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Yong Loo Lin School of Medicine National University of Singapore Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hany","family":"Ariffin","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University Malaya Medical Centre Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shirley K. 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