{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T04:58:04Z","timestamp":1787979484434,"version":"build-2784847793"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,11,22]],"date-time":"2013-11-22T00:00:00Z","timestamp":1385078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Paroxysmal nocturnal haemoglobinuria (\n                    <jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>\n                    ) clones are frequently detected in patients with aplastic anaemia (\n                    <jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>\n                    ). To evaluate the prognostic role of\n                    <jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>\n                    clone presence we conducted a prospective study in 125\n                    <jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>\n                    patients treated with combined immunosuppressive therapy (\n                    <jats:styled-content style=\"fixed-case\">IST<\/jats:styled-content>\n                    ). Seventy\u2010four patients (59%) had a\n                    <jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>\n                    clone (\n                    <jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>\n                    + patients) at diagnosis, with a median clone size of 0\u00b760% in granulocytes and 0\u00b715% in red blood cells. The response rate at 6\u00a0months was higher in\n                    <jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>\n                    + patients than that in\n                    <jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>\n                    \u2010 patients, both after first\u2010 and second\u2010line\n                    <jats:styled-content style=\"fixed-case\">IST<\/jats:styled-content>\n                    : 68% vs. 45%,\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    =\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0\u00b70164 and 53% vs. 13%,\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    =\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0\u00b70502 respectively. Moreover, 42% of\n                    <jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>\n                    + patients achieved complete remission compared with only 16% of\n                    <jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>\n                    \u2010 patients (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    =\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0\u00b70029). In multivariate logistic regression analysis,\n                    <jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>\n                    clone presence (odds ratio 2\u00b756,\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    =\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0\u00b70180) and baseline absolute reticulocyte count (\n                    <jats:styled-content style=\"fixed-case\">ARC<\/jats:styled-content>\n                    ) \u226530\u00a0\u00d7\u00a010\n                    <jats:sup>9<\/jats:sup>\n                    \/l (odds ratio 5\u00b719,\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    =\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0\u00b70011) were independent predictors of response to treatment. Stratification according to\n                    <jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>\n                    positivity and\n                    <jats:styled-content style=\"fixed-case\">ARC<\/jats:styled-content>\n                    \u226530\u00a0\u00d7\u00a010\n                    <jats:sup>9<\/jats:sup>\n                    \/l showed significant distinctions for cumulative incidence of response, overall and failure\u2010free survival. The results of this prospective study confirmed the favourable prognostic value of\n                    <jats:styled-content style=\"fixed-case\">PNH<\/jats:styled-content>\n                    clone presence in the setting of\n                    <jats:styled-content style=\"fixed-case\">IST<\/jats:styled-content>\n                    for\n                    <jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/bjh.12661","type":"journal-article","created":{"date-parts":[[2013,11,22]],"date-time":"2013-11-22T03:55:14Z","timestamp":1385092514000},"page":"546-554","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":100,"title":["Prognostic value of paroxysmal nocturnal haemoglobinuria clone presence in aplastic anaemia patients treated with combined immunosuppression: results of two\u2010centre prospective study"],"prefix":"10.1111","volume":"164","author":[{"given":"Alexander","family":"Kulagin","sequence":"first","affiliation":[{"name":"First Pavlov State Medical University of St. Petersburg St. Petersburg Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Lisukov","sequence":"additional","affiliation":[{"name":"First Pavlov State Medical University of St. Petersburg St. Petersburg Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Ivanova","sequence":"additional","affiliation":[{"name":"First Pavlov State Medical University of St. Petersburg St. Petersburg Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Golubovskaya","sequence":"additional","affiliation":[{"name":"First Pavlov State Medical University of St. Petersburg St. Petersburg Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Kruchkova","sequence":"additional","affiliation":[{"name":"Institute of Clinical Immunology Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Bondarenko","sequence":"additional","affiliation":[{"name":"First Pavlov State Medical University of St. Petersburg St. Petersburg Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Vavilov","sequence":"additional","affiliation":[{"name":"First Pavlov State Medical University of St. Petersburg St. Petersburg Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Stancheva","sequence":"additional","affiliation":[{"name":"First Pavlov State Medical University of St. Petersburg St. Petersburg Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Babenko","sequence":"additional","affiliation":[{"name":"First Pavlov State Medical University of St. Petersburg St. Petersburg Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Sipol","sequence":"additional","affiliation":[{"name":"First Pavlov State Medical University of St. Petersburg St. Petersburg Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Pronkina","sequence":"additional","affiliation":[{"name":"Institute of Clinical Immunology Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Kozlov","sequence":"additional","affiliation":[{"name":"Institute of Clinical Immunology Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Afanasyev","sequence":"additional","affiliation":[{"name":"First 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