{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T20:31:45Z","timestamp":1776198705513,"version":"3.50.1"},"reference-count":53,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,12,16]],"date-time":"2013-12-16T00:00:00Z","timestamp":1387152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2014,3]]},"abstract":"Summary<\/jats:title>The stem cell marker nestin (NES<\/jats:styled-content>) is found in dividing cells of developing and regenerating tissues. Upon terminal differentiation, NES<\/jats:styled-content><\/jats:italic> expression is diminished but may be re\u2010expressed following injury or in cancer. Surprisingly, we recently confirmed NES<\/jats:styled-content> as a tumour\u2010specific marker for mature CD<\/jats:styled-content>138+<\/jats:sup>38+<\/jats:sup> plasma cells (PC<\/jats:styled-content>) in multiple myeloma (MM<\/jats:styled-content>). The present study analysed NES<\/jats:styled-content> expression throughout the spectrum of MM<\/jats:styled-content> developmental stages, starting with individuals with no haematological malignancy, through monoclonal gammopathy of undetermined significance (MGUS<\/jats:styled-content>) and MM<\/jats:styled-content> to plasma cell leukaemia (PCL<\/jats:styled-content>) and MM<\/jats:styled-content> cell lines. NES<\/jats:styled-content> was analysed in bone marrow PC<\/jats:styled-content> of 163 MM<\/jats:styled-content>, four PCL<\/jats:styled-content> and nine MGUS<\/jats:styled-content> patients, 10 individuals with no haematological malignancy and 6 myeloma cell lines (OPM<\/jats:styled-content>\u20102, RPMI<\/jats:styled-content>\u20108226, MOLP<\/jats:styled-content>\u20108, U\u2010266, EJM<\/jats:styled-content>, NCI<\/jats:styled-content>\u2010H929) by flow cytometry and\/or real\u2010time polymerase chain reaction or immunochemistry. We observed a tendency of increased NES<\/jats:styled-content> expression in parallel with disease progression. NES<\/jats:styled-content> was evaluated as a reliable marker for accurate discrimination between MM<\/jats:styled-content> patients and the control group. High NES<\/jats:styled-content> levels were strongly associated with the presence of 1q21 gain. For the first time, NES<\/jats:styled-content> was demonstrated to predict worse response to conventional therapy\/novel agents. These results suggest that NES<\/jats:styled-content> might become a useful clinical parameter with an important role in MM<\/jats:styled-content> pathogenesis.<\/jats:p>","DOI":"10.1111\/bjh.12689","type":"journal-article","created":{"date-parts":[[2013,12,16]],"date-time":"2013-12-16T05:35:15Z","timestamp":1387172115000},"page":"701-709","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Nestin expression throughout multistep pathogenesis of multiple myeloma"],"prefix":"10.1111","volume":"164","author":[{"given":"Hana","family":"Svachova","sequence":"first","affiliation":[{"name":"Babak Myeloma Group Department of Pathological Physiology Faculty of Medicine Masaryk University Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fedor","family":"Kryukov","sequence":"additional","affiliation":[{"name":"Babak Myeloma Group Department of Pathological Physiology Faculty of Medicine 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