{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T22:09:57Z","timestamp":1762898997470},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,1,22]],"date-time":"2014-01-22T00:00:00Z","timestamp":1390348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Accurate genomic characterization requires sufficient amounts of optimal quality <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. An approach for increasing the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> amount is the whole\u2010genome amplification (<jats:styled-content style=\"fixed-case\">WGA<\/jats:styled-content>) method. We applied <jats:styled-content style=\"fixed-case\">WGA<\/jats:styled-content> to the molecular quantification and minimal residual disease (<jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content>) evaluation of acute lymphoblastic leukaemia (<jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content>), aiming to compare the results obtained from genomic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and amplified <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> with <jats:styled-content style=\"fixed-case\">WGA<\/jats:styled-content>, and to evaluate the applicability and the reliability of <jats:styled-content style=\"fixed-case\">WGA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. Twenty paired samples from adult <jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content> patients were sequenced to identify the functional germline <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">J<\/jats:styled-content> segment at diagnosis; real\u2010time quantitative polymerase chain reaction (<jats:styled-content style=\"fixed-case\">RQ<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) quantitative analysis was performed both at diagnosis and follow\u2010up. Genomic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">WGA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> screening identified equivalent 87 rearrangements. At diagnosis, the quantitative evaluation of genomic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> samples showed 1 logarithm difference to <jats:styled-content style=\"fixed-case\">WGA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> samples; these levels are comparable, being within the degree of acceptability and confidence. In the follow\u2010up samples, <jats:styled-content style=\"fixed-case\">RQ<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> analysis on genomic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">WGA<\/jats:styled-content> showed concordant <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content> results in 16\/18 samples, while 2\/18 were <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content>\u2010positive outside the quantitative range by <jats:styled-content style=\"fixed-case\">RQ<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (i.e. &lt;5\u00a0\u00d7\u00a010<jats:sup>\u22125<\/jats:sup>) on genomic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content>\u2010negative on <jats:styled-content style=\"fixed-case\">WGA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">WGA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> enables: (i) the design of accurate targets for <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content> evaluation in <jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content> patients, (ii) accurate disease quantification at diagnosis, (iii) <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content> quantification comparable to genomic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/bjh.12744","type":"journal-article","created":{"date-parts":[[2014,1,22]],"date-time":"2014-01-22T08:21:15Z","timestamp":1390378875000},"page":"341-348","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Whole\u2010genome amplification for the detection of molecular targets and minimal residual disease monitoring in acute lymphoblastic leukaemia"],"prefix":"10.1111","volume":"165","author":[{"given":"Irene","family":"Della Starza","sequence":"first","affiliation":[{"name":"Haematology Department of Cellular Biotechnologies and Haematology \u201cSapienza\u201d University Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia Anna","family":"De Novi","sequence":"additional","affiliation":[{"name":"Haematology Department of Cellular Biotechnologies and Haematology \u201cSapienza\u201d University Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vittorio","family":"Nunes","sequence":"additional","affiliation":[{"name":"Haematology Department of Cellular Biotechnologies and Haematology \u201cSapienza\u201d University Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilaria","family":"Del Giudice","sequence":"additional","affiliation":[{"name":"Haematology Department of Cellular Biotechnologies and Haematology \u201cSapienza\u201d University Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caterina","family":"Ilari","sequence":"additional","affiliation":[{"name":"Haematology Department of Cellular Biotechnologies and Haematology \u201cSapienza\u201d University Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilisa","family":"Marinelli","sequence":"additional","affiliation":[{"name":"Haematology Department of Cellular Biotechnologies and Haematology \u201cSapienza\u201d University Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alina Delia","family":"Negulici","sequence":"additional","affiliation":[{"name":"Haematology Department of Cellular Biotechnologies and Haematology \u201cSapienza\u201d University Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonella","family":"Vitale","sequence":"additional","affiliation":[{"name":"Haematology Department of Cellular Biotechnologies and Haematology \u201cSapienza\u201d University Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabina","family":"Chiaretti","sequence":"additional","affiliation":[{"name":"Haematology Department of Cellular Biotechnologies and Haematology \u201cSapienza\u201d University Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin","family":"Fo\u00e0","sequence":"additional","affiliation":[{"name":"Haematology Department of Cellular Biotechnologies and Haematology \u201cSapienza\u201d University Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Guarini","sequence":"additional","affiliation":[{"name":"Haematology Department of Cellular Biotechnologies and Haematology \u201cSapienza\u201d University Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,1,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1023008921386"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-11-185132"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1976.tb03563.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-5-24"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"8935","DOI":"10.1016\/S0021-9258(18)81883-X","article-title":"Highly efficient DNA synthesis by the phage phi 29 DNA polymerase. Symmetrical mode of DNA replication","volume":"264","author":"Blanco L.","year":"1989","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-01-132837"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-07-2708"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2009.268"},{"key":"e_1_2_8_10_1","first-page":"382","article-title":"Molecular monitoring of childhood acute lymphoblastic leukemia using antigen receptor gene rearrangements and quantitative polymerase chain reaction technology","volume":"90","author":"Cazzaniga G.","year":"2005","journal-title":"Haematologica"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.082089499"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1016\/S0021-9258(18)53833-3","article-title":"Fidelity of phi 29 DNA polymerase. Comparison between protein\u2010primed initiation and DNA polymerization","volume":"268","author":"Esteban J.A.","year":"1993","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.5"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-09-377713"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gah101"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008375931236"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000078004"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2004.01.007"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.659"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0194-19"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2400887"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.20341"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0223(200102)21:2<106::AID-PD983>3.0.CO;2-#"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gnh069"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401245"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2013.204099"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.250"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0029-7844(95)00496-3"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/2.3.209"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.legalmed.2005.05.001"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1447-0756.2009.01110.x"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaq103"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402922"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404586"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404925"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-08-2613"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbjh.12744","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.12744","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T17:05:14Z","timestamp":1696352714000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.12744"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,22]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,5]]}},"alternative-id":["10.1111\/bjh.12744"],"URL":"https:\/\/doi.org\/10.1111\/bjh.12744","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,22]]},"assertion":[{"value":"2013-10-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-12-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}