{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T09:28:12Z","timestamp":1759742892671},"reference-count":62,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,3,12]],"date-time":"2014-03-12T00:00:00Z","timestamp":1394582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>MicroRNAs (miRNAs) are short non\u2010coding RNAs involved in the regulation of gene expression. Selected groups of miRNAs are differentially expressed in various types of cancers. Alterations in miRNAs gene expression have been shown in cells from the B\u2010cell malignancy, multiple myeloma (MM). However, although MM is a disease of plasma cells, abnormalities have been detected in the peripheral blood of the patients.<\/jats:p><jats:p>The goal of our study was to analyse the entire miRNome in peripheral lymphocytes of MM patients using reverse transcription quantitative polymerase chain reaction. Using in silica analysis, we also evaluated some of the most interesting and significant pathways. Analysis revealed that MM samples had a distinct miRNA profile compared to the controls. This resulted in the identification of 203 miRNAs, 85 of which were over\u2010expressed and 118 under\u2010expressed. Of these, 184 possessed validated or highly predicted mRNA targets. We identified 12\u00a0354 mRNA targets of the transcriptome: 36\u00b74% of the related proteins are involved in death processes while the 21% are required for growth and cell proliferation. We have demonstrated that miRNAs are differentially expressed in the peripheral blood of MM patients compared to controls, affecting some pathways involved in the anti\u2010apoptotic process, cell proliferation and maybe anti\u2010angiogenesis.<\/jats:p>","DOI":"10.1111\/bjh.12828","type":"journal-article","created":{"date-parts":[[2014,3,12]],"date-time":"2014-03-12T12:21:55Z","timestamp":1394626915000},"page":"801-813","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["MiRNome expression is deregulated in the peripheral lymphoid compartment of multiple myeloma"],"prefix":"10.1111","volume":"165","author":[{"given":"Salvatore","family":"Campo","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences and Morphological and Functional Images University of Messina Messina Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Allegra","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of General Surgery, Oncology University of Messina Messina Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"D'Ascola","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Morphological and Functional Images University of Messina Messina Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Alonci","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of General Surgery, Oncology University of Messina Messina Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Scuruchi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Morphological and Functional Images University of Messina Messina Italy"},{"name":"Department of Clinical and Experimental Medicine University of Messina Messina Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabina","family":"Russo","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of General Surgery, Oncology University of Messina Messina Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Avenoso","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Morphological and Functional Images University of Messina Messina Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Demetrio","family":"Gerace","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of General Surgery, Oncology University of Messina Messina Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe M.","family":"Campo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Morphological and Functional Images University of Messina Messina Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caterina","family":"Musolino","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of General Surgery, Oncology University of Messina Messina Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,3,12]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"1554","article-title":"MicroRNA expression analysis in multiple myeloma","volume":"106","author":"Masri A.","year":"2005","journal-title":"Blood (ASH Annual Meeting Abstracts)"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2012.1647"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2012.175"},{"key":"e_1_2_8_5_1","first-page":"2472","article-title":"Micro\u2010RNA expression analysis in multiple myeloma plasma cells and cell lines by a quantitative real\u2010time PCR approach [abstract]","volume":"110","author":"Bakkus M.","year":"2007","journal-title":"ASH Annual Meeting"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.26297"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(00)00508-7"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80595-4"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1202629"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V77.12.2688.2688"},{"key":"e_1_2_8_11_1","first-page":"6","article-title":"MicroRNA expression in multiple myeloma is associated with genetic subtype, isotype and survival","volume":"18","author":"Chi J.","year":"2011","journal-title":"Biology Direct"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2007.08.004"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2012.06.038"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009128200"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/01926230701320337"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207116"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2012.08.021"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.5.1265"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3179"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-08-452425"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2009.274"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2005.12.005"},{"key":"e_1_2_8_23_1","doi-asserted-by":"crossref","unstructured":"Hemmings B.A.&Restuccia D.F.(2013)PI3K\u2010PKB\/Akt Pathway.Cold Spring Harb Perspect Biol 2012;http:\/\/cshperspectives.cshlp.org","DOI":"10.1101\/cshperspect.a011189"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.03.013"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.24.0317"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbt.2010.09.006"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2728"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(01)00002-9"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc746"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403084"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-11-3811"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.2174\/156800912802429274"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0268-960X(10)70003-5"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.21959"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00521-X"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.10.5244"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1861409"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.12.029"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0806202105"},{"key":"e_1_2_8_40_1","first-page":"2","article-title":"MicroRNAs: new players in multiple myeloma","volume":"24","author":"Pichiorri F.","year":"2011","journal-title":"Frontiers in Genetics"},{"key":"e_1_2_8_41_1","first-page":"585","article-title":"In multiple myeloma, circulating hyperdiploid B cells have clonotypic immunoglobulin heavy chain rearrangements and may mediate spread of disease","volume":"6","author":"Pilarski L.M.","year":"2000","journal-title":"Clinical Cancer Research"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2007.10.008"},{"key":"e_1_2_8_43_1","doi-asserted-by":"crossref","first-page":"4020","DOI":"10.1182\/blood.V95.12.4020","article-title":"Levels of circulating CD19 cells in patients with multiple myeloma","volume":"95","author":"Rasmussen T.","year":"2000","journal-title":"Blood"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.9.2263"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-01-198408"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-09-178228"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2008.02.005"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.5.1557"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2005.tb00337.x"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2009.10.002"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.5.1.2281"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgs333"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.07.032"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.2174\/156800912802429355"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2006.04.005"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161207780162872"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071553"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1920"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.2741\/4103"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2012.06.317"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-06-222794"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-0124"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.03793.x"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbjh.12828","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.12828","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T07:03:32Z","timestamp":1696316612000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.12828"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,12]]},"references-count":62,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1111\/bjh.12828"],"URL":"https:\/\/doi.org\/10.1111\/bjh.12828","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,12]]},"assertion":[{"value":"2013-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}