{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:13:47Z","timestamp":1790622827928,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,4,28]],"date-time":"2014-04-28T00:00:00Z","timestamp":1398643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    This study aimed to examine the mechanisms of cellular activation by small\u2010size platelet microparticles (\n                    <jats:styled-content style=\"fixed-case\">sPMP<\/jats:styled-content>\n                    ) and to present the performance of high\u2010resolution flow cytometry for the analysis of subcellular entities from different origins. Plasma counts of\n                    <jats:styled-content style=\"fixed-case\">sPMP<\/jats:styled-content>\n                    were analysed in coronary artery disease patients (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a040) and healthy controls (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a040). The effect of\n                    <jats:styled-content style=\"fixed-case\">sPMP<\/jats:styled-content>\n                    and platelet debris (PD) in pathophysiologically relevant doses on platelet and monocyte activation parameters and thrombogenesis was investigated via flow cytometry and thromboelastometry. New generation flow cytometry identifies differences in size, levels and surface molecules of\n                    <jats:styled-content style=\"fixed-case\">sPMP<\/jats:styled-content>\n                    derived in the absence of stimulus, thrombin activation and platelet disruption. Addition of\n                    <jats:styled-content style=\"fixed-case\">sPMP<\/jats:styled-content>\n                    resulted in platelet degranulation and P\u2010selectin redistribution to the membrane (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00\u00b7019) in a dose and time\u2010dependent manner. Blood clotting time decreased after addition of\n                    <jats:styled-content style=\"fixed-case\">sPMP<\/jats:styled-content>\n                    (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00\u00b7005), but was not affected by PD. Blocking P\u2010selectin (CD62P) in\n                    <jats:styled-content style=\"fixed-case\">sPMP<\/jats:styled-content>\n                    markedly reverted the effect on thrombus kinetics (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00\u00b7035). Exposure to\n                    <jats:styled-content style=\"fixed-case\">sPMP<\/jats:styled-content>\n                    stimulated monocyte expression of intercellular adhesion molecule\u20101 (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00\u00b703) and decreased monocyte interleukin\u20106 receptor density (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00\u00b701). These results implicate\n                    <jats:styled-content style=\"fixed-case\">sPMP<\/jats:styled-content>\n                    as a direct source of downstream platelet and monocyte activation. In pathological coronary artery disease conditions, higher levels of\n                    <jats:styled-content style=\"fixed-case\">sPMP<\/jats:styled-content>\n                    favour a prothrombotic state, partly through P\u2010selectin expression.\n                  <\/jats:p>","DOI":"10.1111\/bjh.12913","type":"journal-article","created":{"date-parts":[[2014,7,25]],"date-time":"2014-07-25T10:13:33Z","timestamp":1406283213000},"page":"571-580","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Small\u2010size platelet microparticles trigger platelet and monocyte functionality and modulate thrombogenesis via P\u2010selectin"],"prefix":"10.1111","volume":"166","author":[{"given":"Silvia","family":"Montoro\u2010Garc\u00eda","sequence":"first","affiliation":[{"name":"University of Birmingham Centre for Cardiovascular Sciences City Hospital Birmingham UK"},{"name":"Department of Cardiology Hospital Cl\u00ednico Universitario Virgen de la Arrixaca Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB) Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduard","family":"Shantsila","sequence":"additional","affiliation":[{"name":"University of Birmingham Centre for Cardiovascular Sciences City Hospital Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Hern\u00e1ndez\u2010Romero","sequence":"additional","affiliation":[{"name":"Department of Cardiology Hospital Cl\u00ednico Universitario Virgen de la Arrixaca Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB) Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Jover","sequence":"additional","affiliation":[{"name":"Department of Cardiology Hospital Cl\u00ednico Universitario Virgen de la Arrixaca Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB) Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariano","family":"Vald\u00e9s","sequence":"additional","affiliation":[{"name":"Department of Cardiology Hospital Cl\u00ednico Universitario Virgen de la Arrixaca Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB) Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Mar\u00edn","sequence":"additional","affiliation":[{"name":"Department of Cardiology Hospital Cl\u00ednico Universitario Virgen de la Arrixaca Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB) Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory Y. 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