{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:34:54Z","timestamp":1790008494311,"version":"4.0.1"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,6,7]],"date-time":"2014-06-07T00:00:00Z","timestamp":1402099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"name":"Stichting Sanquin Bloedvoorziening (PPOC grant)","award":["09-025"],"award-info":[{"award-number":["09-025"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2014,9]]},"abstract":"Summary<\/jats:title>\n \n Haemolytic disease of the fetus and newborn (\n HDFN<\/jats:styled-content>\n ) may occur when maternal\n I<\/jats:styled-content>\n g\n G<\/jats:styled-content>\n antibodies against red blood cells (\n RBC<\/jats:styled-content>\n s), often anti\u2010\n R<\/jats:styled-content>\n h\n D<\/jats:styled-content>\n (anti\u2010\n D<\/jats:styled-content>\n ) antibodies, cross the placenta and mediate the destruction of\n RBC<\/jats:styled-content>\n s via phagocytic\n I<\/jats:styled-content>\n g\n G<\/jats:styled-content>\n \u2010\n F<\/jats:styled-content>\n c\u2010receptors (\n F<\/jats:styled-content>\n c\u03b3\n R<\/jats:styled-content>\n ). Clinical severity is not strictly related to titre and is more accurately predicted by the diagnostically\u2010applied monocyte\u2010based antibody\u2010dependent cellular cytotoxicity (\n ADCC<\/jats:styled-content>\n ), a sensitive test with relatively low specificity. This suggests that other factors are involved in the pathogenesis of\n HDFN<\/jats:styled-content>\n . Binding of\n I<\/jats:styled-content>\n g\n G<\/jats:styled-content>\n to\n F<\/jats:styled-content>\n c\u03b3\n R<\/jats:styled-content>\n requires the\n N<\/jats:styled-content>\n \u2010linked glycan at position 297 in the\n I<\/jats:styled-content>\n g\n G<\/jats:styled-content>\n \u2010\n F<\/jats:styled-content>\n c\u2010region, consisting of several different glycoforms. We therefore systematically analysed\n I<\/jats:styled-content>\n g\n G<\/jats:styled-content>\n \u2010derived glycopeptides by mass spectrometry from 70 anti\u2010\n D I<\/jats:styled-content>\n g\n G<\/jats:styled-content>\n 1 antibodies purified from the plasma of alloimmunized pregnant women. This revealed a variable decrease in\n F<\/jats:styled-content>\n c\u2010fucosylation in the majority of anti\u2010\n D I<\/jats:styled-content>\n g\n G<\/jats:styled-content>\n 1 (even down to 12%), whereas the total\n I<\/jats:styled-content>\n g\n G<\/jats:styled-content>\n of these patients remained highly fucosylated, like in healthy individuals (>90%). The degree of anti\u2010D fucosylation correlated significantly with\n CD<\/jats:styled-content>\n 16 (\n F<\/jats:styled-content>\n c\u03b3\n RIII<\/jats:styled-content>\n a)\u2010mediated\n ADCC<\/jats:styled-content>\n , in agreement with increased affinity of defucosylated\n I<\/jats:styled-content>\n g\n G<\/jats:styled-content>\n to human\n F<\/jats:styled-content>\n c\u03b3\n RIII<\/jats:styled-content>\n a. Additionally, low anti\u2010\n D<\/jats:styled-content>\n fucosylation correlated significantly with low fetal\u2010neonatal haemoglobin levels, thus with increased haemolysis, suggesting\n I<\/jats:styled-content>\n g\n G<\/jats:styled-content>\n \u2010fucosylation to be an important pathological feature in\n HDFN<\/jats:styled-content>\n with diagnostic potential.\n <\/jats:p>","DOI":"10.1111\/bjh.12965","type":"journal-article","created":{"date-parts":[[2014,6,7]],"date-time":"2014-06-07T03:27:19Z","timestamp":1402111639000},"page":"936-945","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":128,"title":["Low anti\u2010\n R<\/scp>\n h\n D I<\/scp>\n g\n G<\/scp>\n \u2010\n F<\/scp>\n c\u2010fucosylation in pregnancy: a new variable predicting severity in haemolytic disease of the fetus and newborn"],"prefix":"10.1111","volume":"166","author":[{"given":"Rick","family":"Kapur","sequence":"first","affiliation":[{"name":"Department of Experimental Immunohaematology, Sanquin Research, Amsterdam and Landsteiner Laboratory Academic Medical Centre University of Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana","family":"Della Valle","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohaematology, Sanquin Research, Amsterdam and Landsteiner Laboratory Academic Medical Centre University of Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myrthe","family":"Sonneveld","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohaematology, Sanquin Research, Amsterdam and Landsteiner Laboratory Academic Medical Centre University of Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes","family":"Hipgrave Ederveen","sequence":"additional","affiliation":[{"name":"Centre for Proteomics and Metabolomics Leiden University Medical Centre Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remco","family":"Visser","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohaematology, Sanquin Research, Amsterdam and Landsteiner Laboratory Academic Medical Centre University of Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Ligthart","sequence":"additional","affiliation":[{"name":"Erythrocyte Serology Sanquin Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masja","family":"de Haas","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohaematology, Sanquin Research, Amsterdam and Landsteiner Laboratory Academic Medical Centre University of Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Wuhrer","sequence":"additional","affiliation":[{"name":"Centre for Proteomics and Metabolomics Leiden University Medical Centre Leiden The Netherlands"},{"name":"Division of BioAnalytical Chemistry VU University Amsterdam The Netherlands"},{"name":"Department of Molecular Cell Biology and Immunology VU University Medical Centre Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Ellen","family":"van der Schoot","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohaematology, Sanquin Research, Amsterdam and Landsteiner Laboratory Academic Medical Centre University of Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gestur","family":"Vidarsson","sequence":"additional","affiliation":[{"name":"Department of Experimental Immunohaematology, Sanquin Research, Amsterdam and Landsteiner Laboratory Academic Medical Centre University of Amsterdam Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,6,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI65708"},{"key":"e_1_2_8_3_1","first-page":"1379","article-title":"Immunoglobulin G glycosylation and clinical outcome in rheumatoid arthritis during pregnancy","volume":"27","author":"Alavi A.","year":"2000","journal-title":"Journal of Rheumatology"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1154315"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-03-0989"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr300887z"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1996.d01-741.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1996.0104"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr400589m"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2004.12.013"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10719-012-9381-6"},{"key":"e_1_2_8_12_1","first-page":"328","article-title":"Current status of immunoprophylaxis with anti\u2010D immunoglobin","volume":"85","author":"Engelfriet C.P.","year":"2003","journal-title":"Vox Sanguinis"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M510171200"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1108455108"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2892"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2909"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302488"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-3704"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1129594"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2014.01.015"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-09-527978"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10719-011-9364-z"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.01742.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1246-7820(01)00215-4"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2003.02119.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1178"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2007.02.005"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-11-3929"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2011.01552.x"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2005.08.009"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2001.112970"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/316452a0"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-8411(89)90148-0"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-144600"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-8411(91)90173-A"},{"key":"e_1_2_8_36_1","first-page":"513","article-title":"Antibody\u2010mediated erythrolysis and erythrophagocytosis by human monocytes, macrophages and activated macrophages. 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