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Previously, we discovered a potential therapeutic molecular target, <jats:styled-content style=\"fixed-case\">MDX<\/jats:styled-content>3 (<jats:styled-content style=\"fixed-case\">MAX<\/jats:styled-content> dimerization protein 3), in preB <jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content>. In this study, we hypothesize that an effective si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> therapy for preB <jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content> can be developed using anti<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>22 antibody (\u03b1<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>22 Ab) and nanoparticles. We composed nanocomplexes with super paramagnetic iron oxide nanoparticles (<jats:styled-content style=\"fixed-case\">SPIO NP<\/jats:styled-content>s), \u03b1<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>22 Abs and <jats:styled-content style=\"fixed-case\">MXD<\/jats:styled-content>3 si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> molecules based on physical interactions between the molecules. We demonstrated that the <jats:styled-content style=\"fixed-case\">MXD<\/jats:styled-content>3 si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010\u03b1<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>22 Ab\u2010<jats:styled-content style=\"fixed-case\">SPIO NP<\/jats:styled-content> complexes entered leukaemia cells and knocked down <jats:styled-content style=\"fixed-case\">MXD<\/jats:styled-content>3, leading the cells to undergo apoptosis and resulting in decreased live cell counts in the cell line Reh and in primary preB <jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content> samples <jats:italic>in vitro<\/jats:italic>. Furthermore, the cytotoxic effects of the <jats:styled-content style=\"fixed-case\">MXD<\/jats:styled-content>3 si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010\u03b1<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>22 Ab\u2010<jats:styled-content style=\"fixed-case\">SPIO NP<\/jats:styled-content> complexes were significantly enhanced by addition of the chemotherapy drugs vincristine or doxorubicin. We also ruled out potential cytotoxic effects of the <jats:styled-content style=\"fixed-case\">MXD<\/jats:styled-content>3 si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010\u03b1<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>22 Ab\u2010<jats:styled-content style=\"fixed-case\">SPIO NP<\/jats:styled-content> complexes on normal primary haematopoietic cells. Normal B cells were affected while <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34\u2010positive haematopoietic stem cells and non\u2010B cells were not. These data suggest that <jats:styled-content style=\"fixed-case\">MXD<\/jats:styled-content>3 si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010\u03b1<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>22 Ab\u2010<jats:styled-content style=\"fixed-case\">SPIO NP<\/jats:styled-content> complexes have the potential to be a new targeted therapy for preB <jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/bjh.13066","type":"journal-article","created":{"date-parts":[[2014,9,8]],"date-time":"2014-09-08T07:06:22Z","timestamp":1410159982000},"page":"487-499","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Targeted therapy with <scp>MXD<\/scp>3 si<scp>RNA<\/scp>, anti\u2010<scp>CD<\/scp>22 antibody and nanoparticles for precursor <scp>B<\/scp>\u2010cell acute lymphoblastic leukaemia"],"prefix":"10.1111","volume":"167","author":[{"given":"Noriko","family":"Satake","sequence":"first","affiliation":[{"name":"Department of Pediatrics University of California Davis Sacramento CA USA"},{"name":"Stem Cell Program and Institute for Regenerative Cures University of California Davis Sacramento CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Connie","family":"Duong","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of California Davis Sacramento CA USA"},{"name":"Stem Cell Program and Institute for Regenerative Cures University of California Davis Sacramento CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cathy","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of California Davis Sacramento CA USA"},{"name":"Stem Cell Program and Institute for Regenerative Cures University of California Davis Sacramento CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo A.","family":"Barisone","sequence":"additional","affiliation":[{"name":"Department of Pharmacology University of California Davis Sacramento CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elva","family":"Diaz","sequence":"additional","affiliation":[{"name":"Department of Pharmacology University of California Davis Sacramento CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Tuscano","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine University of California Davis Sacramento CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Rocke","sequence":"additional","affiliation":[{"name":"Departments of Public Health Sciences and Biomedical Engineering University of California Davis Sacramento CA 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