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Thus, we investigated <jats:italic>HMGA2 <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression in 66 patients with MPNs including 23 polycythaemia vera (PV), 33 essential thrombocythaemia (ET) and 10 primary myelofibrosis (PMF). <jats:italic>HMGA2 <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression, especially variant 1 with 3\u2032UTR that contains <jats:italic>MIRLET7<\/jats:italic>\u2010specific sites, rather than variant 2 lacking 3\u2032UTR, is frequently deregulated due to decreased <jats:italic>MIRLET7<\/jats:italic> expression in granulocytes from over 20% of PV and ET, and in either granulocytes or CD34<jats:sup>+<\/jats:sup> cells from 100% of PMF. Patients with deregulated <jats:italic>HMGA2 <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression were significantly more likely to show splenomegaly, high serum lactate dehydrogenase values, and methylation of the <jats:italic>CDKN2A<\/jats:italic> promoter compared with other patients without deregulation of <jats:italic>HMGA2<\/jats:italic>. A histone deacetylase inhibitor, panobinostat, significantly increased <jats:italic>MIRLET7<\/jats:italic> expression and reduced variant 1 of <jats:italic>HMGA2 <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression, but not variant 2, in both U937 cells and PMF\u2010derived CD34<jats:sup>+<\/jats:sup> cells. Moreover, both panobinostat and small interfering RNA of <jats:italic>HMGA2<\/jats:italic> demethylated the <jats:italic>CDKN2A<\/jats:italic> promoter in U937 cells. In conclusion, the frequently dysregulated <jats:italic>MIRLET7<\/jats:italic>\/<jats:italic>HMGA2<\/jats:italic> axis could be a therapeutic target in MPNs.<\/jats:p>","DOI":"10.1111\/bjh.13129","type":"journal-article","created":{"date-parts":[[2014,9,19]],"date-time":"2014-09-19T06:05:18Z","timestamp":1411106718000},"page":"338-349","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Dysregulation of the <i><scp>MIRLET<\/scp>7<\/i>\/<i><scp>HMGA<\/scp>2<\/i> axis with methylation of the <i><scp>CDKN<\/scp>2A<\/i> promoter in myeloproliferative neoplasms"],"prefix":"10.1111","volume":"168","author":[{"given":"Kayo","family":"Harada\u2010Shirado","sequence":"first","affiliation":[{"name":"Department of Cardiology and Hematology Fukushima Medical University Fukushima 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