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Following 7 and 14\u00a0d in culture, early\u2010 and late\u2010 erythroblasts were isolated and purified. No morphological difference in maturation was observed following 7\u00a0d in culture, while a delayed maturation was observed in the patient group after 14\u00a0d. Following <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> isolation and linear amplification, gene expression analyses were performed using microarray technology. The generated data were analysed by two methods: the <jats:styled-content style=\"fixed-case\">BRB<\/jats:styled-content>\u2010ArrayTools platform and the Bioconductor platform using bead level data. Following 7\u00a0d culture, there was no difference in gene expression between the control and patient groups. Following 14\u00a0d culture, 384 differentially expressed genes were identified by either analysis. A subset of 90 genes was selected and the results were confirmed by Quantitative\u2010Real\u2010Time\u2010polymerase chain reaction. Pathways shown to be significantly altered in the patient group include apoptosis, <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content>inase and the nuclear factor\u2010\u03baB pathway.<\/jats:p>","DOI":"10.1111\/bjh.13432","type":"journal-article","created":{"date-parts":[[2015,4,20]],"date-time":"2015-04-20T05:04:58Z","timestamp":1429506298000},"page":"257-267","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Differential gene expression analysis in early and late erythroid progenitor cells in \u03b2\u2010thalassaemia"],"prefix":"10.1111","volume":"170","author":[{"given":"Luke","family":"Forster","sequence":"first","affiliation":[{"name":"School of Pathology and Laboratory Medicine University of Western Australia Nedlands WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"McCooke","sequence":"additional","affiliation":[{"name":"Centre for Comparative 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