{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:14:22Z","timestamp":1792041262599,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,7,24]],"date-time":"2015-07-24T00:00:00Z","timestamp":1437696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Programme Hospitalier de Recherche Clinique","award":["PHRC 2009-18"],"award-info":[{"award-number":["PHRC 2009-18"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The level of circulating platelet\u2010, erythrocyte\u2010, leucocyte\u2010 and endothelial\u2010derived microparticles detected by high\u2010sensitivity flow cytometry was investigated in 37 \u03b2\u2010thalassaemia major patients receiving a regular transfusion regimen. The phospholipid procoagulant potential of the circulating microparticles and the microparticle\u2010dependent tissue factor activity were evaluated. A high level of circulating erythrocyte\u2010 and platelet\u2010microparticles was found. In contrast, the number of endothelial microparticles was within the normal range. Platelet microparticles were significantly higher in splenectomized than in non\u2010splenectomized patients, independent of platelet count (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0\u00b7001). Multivariate analysis indicated that phospholipid\u2010dependent procoagulant activity was influenced by both splenectomy (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    =\u00a00\u00b7001) and platelet microparticle level (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0\u00b7001). Erythrocyte microparticles were not related to splenectomy, appear to be devoid of proper procoagulant activity and no relationship between their production and haemolysis, dyserythropoiesis or oxidative stress markers could be established. Intra\u2010microparticle labelling with anti\u2010HbF antibodies showed that they originate only partially (median of 28%) from thalassaemic erythropoiesis. In conclusion, when \u03b2\n                    <jats:italic>\u2010<\/jats:italic>\n                    thalassaemia major patients are intensively transfused, the procoagulant activity associated with thalassaemic erythrocyte microparticles is probably diluted by transfusions. In contrast, platelet microparticles, being both more elevated and more procoagulant, especially after splenectomy, may contribute to the residual thrombotic risk reported in splenectomized multi\u2010transfused \u03b2\u2010thalassaemia major patients.\n                  <\/jats:p>","DOI":"10.1111\/bjh.13609","type":"journal-article","created":{"date-parts":[[2015,7,24]],"date-time":"2015-07-24T02:19:58Z","timestamp":1437704398000},"page":"615-624","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Platelet and not erythrocyte microparticles are procoagulant in transfused thalassaemia major patients"],"prefix":"10.1111","volume":"171","author":[{"given":"Imane","family":"Agouti","sequence":"first","affiliation":[{"name":"Centre de R\u00e9f\u00e9rence Maladies Rares Thalass\u00e9mies, Marseille\u2010Lyon Service d'H\u00e9mato\u2010Oncologie P\u00e9diatrique H\u00f4pital de la Timone APHM Marseille 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