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A proteomic approach was used to compare profiles of the B\u2010lineage <jats:styled-content style=\"fixed-case\">ALL GC<\/jats:styled-content>\u2010sensitive cell line, PreB 697, and its <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>\u2010resistant sub\u2010line, R3F9, pre\u2010 and post\u2010dexamethasone exposure. <jats:styled-content style=\"fixed-case\">PAX<\/jats:styled-content>5, a transcription factor critical to B\u2010cell development was differentially regulated in the PreB 697 compared to the R3F9 cell line in response to <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">PAX<\/jats:styled-content>5 basal protein expression was less in R3F9 compared to its <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>\u2010sensitive parent and confirmed to be lower in other <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>\u2010resistant sub\u2010lines of Pre B 697 and was associated with a decreased expression of the <jats:styled-content style=\"fixed-case\">PAX<\/jats:styled-content>5 transcriptional target, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>19. Gene set enrichment analysis showed that increasing <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>\u2010resistance was associated with differentiation from preB\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> to an immature B\u2010lymphocyte stage. <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>\u2010resistant sub\u2010lines were shown to have higher levels of phosphorylated <jats:styled-content style=\"fixed-case\">JNK<\/jats:styled-content> compared to the parent line and <jats:styled-content style=\"fixed-case\">JNK<\/jats:styled-content> inhibition caused re\u2010sensitization to <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>. Exploiting this maturation may be key to overcoming <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> resistance and targeting signalling pathways linked to the maturation state, such as <jats:styled-content style=\"fixed-case\">JNK<\/jats:styled-content>, may be a novel approach.<\/jats:p>","DOI":"10.1111\/bjh.13647","type":"journal-article","created":{"date-parts":[[2015,8,27]],"date-time":"2015-08-27T06:43:41Z","timestamp":1440657821000},"page":"595-605","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Quantitative proteomic analysis reveals maturation as a mechanism underlying glucocorticoid resistance in B lineage <scp>ALL<\/scp> and re\u2010sensitization by <scp>JNK<\/scp> inhibition"],"prefix":"10.1111","volume":"171","author":[{"given":"Lindsay","family":"Nicholson","sequence":"first","affiliation":[{"name":"Newcastle Cancer Centre at the Northern Institute for Cancer Research Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline A.","family":"Evans","sequence":"additional","affiliation":[{"name":"Stem Cell and Leukaemia Proteomics Laboratory School of Cancer and Enabling Sciences Manchester Academic Health Science Centre University of Manchester Manchester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Matheson","sequence":"additional","affiliation":[{"name":"Newcastle Cancer Centre at the Northern Institute for Cancer Research Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynne","family":"Minto","sequence":"additional","affiliation":[{"name":"Newcastle Cancer Centre at the Northern Institute for Cancer Research Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Keilty","sequence":"additional","affiliation":[{"name":"Newcastle Cancer Centre at the Northern Institute for Cancer Research Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryna","family":"Sanichar","sequence":"additional","affiliation":[{"name":"Newcastle Cancer Centre at the Northern Institute for Cancer Research Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marian","family":"Case","sequence":"additional","affiliation":[{"name":"Newcastle Cancer Centre at the Northern Institute for Cancer Research Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Schwab","sequence":"additional","affiliation":[{"name":"Newcastle Cancer Centre at the Northern Institute for Cancer Research Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Williamson","sequence":"additional","affiliation":[{"name":"Newcastle Cancer Centre at the Northern Institute for Cancer Research Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes","family":"Rainer","sequence":"additional","affiliation":[{"name":"Tyrolean Cancer Research Institute Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine J.","family":"Harrison","sequence":"additional","affiliation":[{"name":"Newcastle Cancer Centre at the Northern Institute for Cancer Research Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"Kofler","sequence":"additional","affiliation":[{"name":"Tyrolean Cancer Research Institute Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew G.","family":"Hall","sequence":"additional","affiliation":[{"name":"Newcastle Cancer Centre at the Northern Institute for Cancer Research Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher P. 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