{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:03:40Z","timestamp":1791169420004,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,10,22]],"date-time":"2015-10-22T00:00:00Z","timestamp":1445472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007313","name":"University of Texas MD Anderson Cancer Center","doi-asserted-by":"publisher","award":["CA016672"],"award-info":[{"award-number":["CA016672"]}],"id":[{"id":"10.13039\/100007313","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2016,2]]},"abstract":"Summary<\/jats:title>The prognostic value of minimal residual disease (MRD<\/jats:styled-content>) assessed by multi\u2010parameter flow cytometry (MFC<\/jats:styled-content>) was investigated among 340 adult patients with B\u2010cell acute lymphoblastic leukaemia (B\u2010ALL<\/jats:styled-content>) treated between\u00a02004 and 2014 using regimens including the hyperCVAD<\/jats:styled-content> (hyperfractionated cyclophosphamide, vincristine, doxorubicin, dexamethasone, methotrexate, cytarabine) backbone. Among them, 323 (95%) achieved complete remission (CR<\/jats:styled-content>) and were included in this study. Median age was 52\u00a0years (range, 15\u201384). Median white blood cell count (WBC<\/jats:styled-content>) was 9\u00b735\u00a0\u00d7\u00a0109<\/jats:sup>\/l (range, 0\u00b74\u2013658\u00b71\u00a0\u00d71\u00a009<\/jats:sup>\/l). MRD<\/jats:styled-content> by MFC<\/jats:styled-content> was initially assessed with a sensitivity of 0\u00b701%, using a 15\u2010marker, 4\u2010colour panel and subsequently a 6\u2010colour panel on bone marrow specimens obtained at CR<\/jats:styled-content> achievement and at approximately 3\u00a0month intervals thereafter. MRD<\/jats:styled-content> negative status at CR<\/jats:styled-content> was associated with improved disease\u2010free survival (DFS<\/jats:styled-content>) and overall survival (OS<\/jats:styled-content>) (P<\/jats:italic>\u00a0=\u00a00\u00b7004 and P<\/jats:italic>\u00a0=\u00a00\u00b703, respectively). Similarly, achieving MRD<\/jats:styled-content> negative status at approximately 3 and 6\u00a0months was associated with improved DFS<\/jats:styled-content> (P<\/jats:italic>\u00a0=\u00a00\u00b7004 and P<\/jats:italic>\u00a0<\u00a00\u00b70001, respectively) and OS<\/jats:styled-content> (P<\/jats:italic>\u00a0=\u00a00\u00b7004 and P<\/jats:italic>\u00a0<\u00a00\u00b70001, respectively). Multivariate analysis including age, WBC<\/jats:styled-content> at presentation, cytogenetics (standard versus<\/jats:italic> high risk) and MRD<\/jats:styled-content> status at CR<\/jats:styled-content>, 3 and 6\u00a0months, indicated that MRD<\/jats:styled-content> negative status at CR<\/jats:styled-content> was an independent predictor of DFS<\/jats:styled-content> (P<\/jats:italic>\u00a0<\u00a00\u00b705). Achievement of an MRD<\/jats:styled-content> negative state assessed by MFC<\/jats:styled-content> is an important predictor of DFS<\/jats:styled-content> and OS<\/jats:styled-content> in adult patients with ALL<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/bjh.13834","type":"journal-article","created":{"date-parts":[[2015,10,23]],"date-time":"2015-10-23T13:39:05Z","timestamp":1445607545000},"page":"392-400","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":108,"title":["Minimal residual disease assessed by multi\u2010parameter flow cytometry is highly prognostic in adult patients with acute lymphoblastic leukaemia"],"prefix":"10.1111","volume":"172","author":[{"given":"Farhad","family":"Ravandi","sequence":"first","affiliation":[{"name":"Departments of Leukemia The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey 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