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Currently, there are no known specific biomarkers for <jats:styled-content style=\"fixed-case\">ITP<\/jats:styled-content> to support differential diagnosis and treatment decisions. Profiling of serum proteins may be valuable for identifying such biomarkers. Sera from 46 patients with primary chronic <jats:styled-content style=\"fixed-case\">ITP<\/jats:styled-content> and 34 healthy blood donors were analysed using a microarray of 755 antibodies. We identified 161 differentially expressed proteins. In addition to oncoproteins and tumour\u2010suppressor proteins, including apoptosis regulator <jats:styled-content style=\"fixed-case\">BCL<\/jats:styled-content>2, breast cancer type 1 susceptibility protein (<jats:styled-content style=\"fixed-case\">BRCA<\/jats:styled-content>1), Fanconi anaemia complementation group C (<jats:styled-content style=\"fixed-case\">FANCC<\/jats:styled-content>) and vascular endothelial growth factor A (<jats:styled-content style=\"fixed-case\">VEGFA<\/jats:styled-content>), we detected six anti\u2010nuclear autoantibodies in a subset of <jats:styled-content style=\"fixed-case\">ITP<\/jats:styled-content> patients: anti\u2010<jats:styled-content style=\"fixed-case\">PCNA<\/jats:styled-content>, anti\u2010SmD, anti\u2010Ro\/<jats:styled-content style=\"fixed-case\">SSA<\/jats:styled-content>60, anti\u2010Ro\/<jats:styled-content style=\"fixed-case\">SSA<\/jats:styled-content>52, anti\u2010La\/<jats:styled-content style=\"fixed-case\">SSB<\/jats:styled-content> and anti\u2010<jats:styled-content style=\"fixed-case\">RNPC<\/jats:styled-content> antibodies. This finding may provide a rational explanation for the association of <jats:styled-content style=\"fixed-case\">ITP<\/jats:styled-content> with malignancies and other autoimmune diseases. While <jats:italic><jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>1<\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression in the peripheral blood mononuclear cells (<jats:styled-content style=\"fixed-case\">PBMC<\/jats:styled-content>) of patients was significantly downregulated, an accumulation of <jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>1 protein was observed in the platelets of <jats:styled-content style=\"fixed-case\">ITP<\/jats:styled-content> patients. This may indicate dysregulation of <jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>1 expression in <jats:styled-content style=\"fixed-case\">PBMC<\/jats:styled-content> and megakaryocytes and may lead to an imbalanced immune response and impaired thrombopoiesis. In conclusion, we provide novel insights into the pathogenic mechanisms of <jats:styled-content style=\"fixed-case\">ITP<\/jats:styled-content> that warrant further exploration.<\/jats:p>","DOI":"10.1111\/bjh.13861","type":"journal-article","created":{"date-parts":[[2015,12,2]],"date-time":"2015-12-02T07:57:05Z","timestamp":1449043025000},"page":"602-615","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Identification of novel biomarkers in chronic immune thrombocytopenia (<scp>ITP<\/scp>) by microarray\u2010based serum protein profiling"],"prefix":"10.1111","volume":"172","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0868-4059","authenticated-orcid":false,"given":"G\u00fcrkan","family":"Bal","sequence":"first","affiliation":[{"name":"Institute for Transfusion Medicine Charit\u00e9 Universit\u00e4tsmedizin Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias E.","family":"Futschik","sequence":"additional","affiliation":[{"name":"Center for Biomedical Research Universidade do Algarve Faro Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Hartl","sequence":"additional","affiliation":[{"name":"Berlin\u2010Brandenburg Centre for Regenerative Therapies (BCRT) Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frauke","family":"Ringel","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine Charit\u00e9 Universit\u00e4tsmedizin Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian","family":"Kamhieh\u2010Milz","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine Charit\u00e9 Universit\u00e4tsmedizin Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktor","family":"Sterzer","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine Charit\u00e9 Universit\u00e4tsmedizin Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rg D.","family":"Hoheisel","sequence":"additional","affiliation":[{"name":"Division of Functional Genome Analysis Deutsches Krebsforschungszentrum Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed S. 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