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The applicability of this technology is limited by the need of a standard curve based on diagnostic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. The digital droplet <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (dd<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) technology has been recently applied to various medical fields, but its use in <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content> monitoring is under investigation. In this study, we analysed 50 <jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content> cases by both methods in two phases: in the first, we established analytical parameters to investigate the applicability of this new technique; in the second, we analysed <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content> levels in 141 follow\u2010up (<jats:styled-content style=\"fixed-case\">FU<\/jats:styled-content>) samples to investigate the possible use of dd<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> for <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content> monitoring in <jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content> patients. We documented that dd<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> has sensitivity and accuracy at least comparable to those of <jats:styled-content style=\"fixed-case\">RQ<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Overall, the two methods gave concordant results in 124 of the 141 analysed <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content> samples (88%, <jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b794). Discordant results were found in 12% borderline cases.<\/jats:p><jats:p>The results obtained prove that dd<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> is a reliable method for <jats:styled-content style=\"fixed-case\">MRD<\/jats:styled-content> monitoring in <jats:styled-content style=\"fixed-case\">ALL<\/jats:styled-content>, with the advantage of quantifying without the need of the calibration curves. Its application in a cohort of patients with a longer <jats:styled-content style=\"fixed-case\">FU<\/jats:styled-content> will conclusively define its clinical predictive value.<\/jats:p>","DOI":"10.1111\/bjh.14082","type":"journal-article","created":{"date-parts":[[2016,5,13]],"date-time":"2016-05-13T00:07:40Z","timestamp":1463098060000},"page":"541-549","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Comparative analysis between RQ\u2010PCR and digital\u2010droplet\u2010PCR of immunoglobulin\/T\u2010cell receptor gene rearrangements to monitor minimal residual disease in acute lymphoblastic leukaemia"],"prefix":"10.1111","volume":"174","author":[{"given":"Irene","family":"Della Starza","sequence":"first","affiliation":[{"name":"Department of Cellular Biotechnologies and Haematology \u201cSapienza\u201d University of Rome Rome 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