{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T02:36:50Z","timestamp":1787971010093,"version":"build-2784847793"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,9,8]],"date-time":"2016-09-08T00:00:00Z","timestamp":1473292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R43HL123670"],"award-info":[{"award-number":["R43HL123670"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p>Sickle cell disease (<jats:styled-content style=\"fixed-case\">SCD<\/jats:styled-content>) is a significant healthcare burden worldwide, but most affected individuals reside in low\u2010resource areas where access to diagnostic testing may be limited. We developed and validated a rapid, inexpensive, disposable diagnostic test, the HemoType<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content><jats:sup>\u2122<\/jats:sup>, based on novel monoclonal antibodies (<jats:styled-content style=\"fixed-case\">MA<\/jats:styled-content>bs) that differentiate normal adult haemoglobin (Hb A), sickle haemoglobin (Hb S) and haemoglobin C (Hb C). In competitive enzyme\u2010linked immunosorbent assays, each <jats:styled-content style=\"fixed-case\">MA<\/jats:styled-content>b bound only its target with &lt;0\u00b71% cross\u2010reactivity. With the HemoType<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content><jats:sup>\u2122<\/jats:sup> test procedure, the sensitivity for each variant was &lt;5\u00b70\u00a0g\/l. The accuracy of HemoType<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content><jats:sup>\u2122<\/jats:sup> was evaluated on 100 whole blood samples from individuals with common relevant haemoglobin phenotypes, including normal (Hb <jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>,<jats:italic> N<\/jats:italic>\u00a0=\u00a020), carrier or trait (Hb <jats:styled-content style=\"fixed-case\">AS<\/jats:styled-content>,<jats:italic> N<\/jats:italic>\u00a0=\u00a022; Hb <jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>,<jats:italic> N<\/jats:italic>\u00a0=\u00a020), <jats:styled-content style=\"fixed-case\">SCD<\/jats:styled-content> (Hb <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>,<jats:italic> N<\/jats:italic>\u00a0=\u00a022; Hb <jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>,<jats:italic> N<\/jats:italic>\u00a0=\u00a013), and Hb C disease (Hb <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>,<jats:italic> N<\/jats:italic>\u00a0=\u00a03). The correct haemoglobin phenotype was identified in 100% of these samples. The accuracy of the test was not affected by Hb F (0\u201394\u00b78% of total Hb) or Hb A<jats:sub>2<\/jats:sub> (0\u20135\u00b76% of total Hb). HemoType<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content><jats:sup>\u2122<\/jats:sup> requires &lt;1\u00a0\u03bcl of whole blood and no instruments or power sources. The total time\u2010to\u2010result is &lt;20\u00a0min. HemoType<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content><jats:sup>\u2122<\/jats:sup> may be a practical solution for point\u2010of\u2010care testing for <jats:styled-content style=\"fixed-case\">SCD<\/jats:styled-content> and carrier status in low\u2010resource settings.<\/jats:p>","DOI":"10.1111\/bjh.14298","type":"journal-article","created":{"date-parts":[[2016,9,8]],"date-time":"2016-09-08T06:34:42Z","timestamp":1473316482000},"page":"724-732","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["A rapid, inexpensive and disposable point\u2010of\u2010care blood test for sickle cell disease using novel, highly specific monoclonal antibodies"],"prefix":"10.1111","volume":"175","author":[{"given":"Charles T.","family":"Quinn","sequence":"first","affiliation":[{"name":"Cincinnati Children's Hospital Medical Center Cincinnati OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary C.","family":"Paniagua","sequence":"additional","affiliation":[{"name":"Cincinnati Children's Hospital Medical Center Cincinnati OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert K.","family":"DiNello","sequence":"additional","affiliation":[{"name":"Silver Lake Research Corporation Azusa CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anand","family":"Panchal","sequence":"additional","affiliation":[{"name":"Silver Lake Research Corporation Azusa CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Geisberg","sequence":"additional","affiliation":[{"name":"Silver Lake Research Corporation Azusa CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.1594"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2013-303773"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M309545200"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1996.tb00484.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-8-271"},{"key":"e_1_2_7_7_1","volume-title":"Antibodies: A Laboratory Manual","author":"Greenfield E.A.","year":"2013"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amepre.2011.09.013"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630268508997010"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-015-0473-6"},{"key":"e_1_2_7_11_1","volume-title":"ELISA and Other Solid Phase Immunoassays New York","author":"Kemeny D.M.","year":"1988"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1414739111"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.5581\/1516-8484.20110015"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2015.12.002"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24232"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijans.2015.08.002"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-3661-4"},{"key":"e_1_2_7_18_1","first-page":"5","article-title":"An up\u2010date on the prevalence of sickle cell trait in Eastern and Western Uganda","volume":"10","author":"Okwi A.L.","year":"2010","journal-title":"BMC Blood Disorders"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1104"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61229-X"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0144901"},{"key":"e_1_2_7_22_1","unstructured":"Prahl S.(2012)Tabulated molar extinction coefficient for hemoglobin in water. Available at:http:\/\/omlc.org\/spectra\/hemoglobin\/summary.html(Accessed 21 June 2016)."},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-07-233700"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3109\/03630269509036939"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)61575-7"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.10937"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.puhe.2007.12.005"},{"key":"e_1_2_7_28_1","volume-title":"Lateral Flow Immunoassay Totowa","author":"Wong R.","year":"2008"},{"key":"e_1_2_7_29_1","unstructured":"World Health Organization. (2010)Sickle cell disease: A strategy for the WHO African Region. Available athttp:\/\/www.afro.who.int\/en\/clusters-a-programmes\/dpc\/non-communicable-diseases-managementndm\/npc-publications.html(Accessed 21 June 2016)."},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3lc41302k"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbjh.14298","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbjh.14298","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.14298","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T16:25:34Z","timestamp":1696350334000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.14298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,8]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1111\/bjh.14298"],"URL":"https:\/\/doi.org\/10.1111\/bjh.14298","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,8]]},"assertion":[{"value":"2016-04-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-06-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-09-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}