{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T18:36:30Z","timestamp":1780598190174,"version":"3.54.1"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,10,6]],"date-time":"2016-10-06T00:00:00Z","timestamp":1475712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000982","name":"Conquer Cancer Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000982","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Next\u2010generation sequencing (<jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>)\u2010based circulating tumour <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> (ct<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>) detection is a promising monitoring tool for lymphoid malignancies. We evaluated whether the presence of ct<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> was associated with outcome after allogeneic haematopoietic stem cell transplantation (<jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content>) in lymphoma patients. We studied 88 patients drawn from a phase 3 clinical trial of reduced\u2010intensity conditioning <jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content> in lymphoma. Conventional restaging and collection of peripheral blood samples occurred at pre\u2010specified time points before and after <jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content> and were assayed for ct<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> by sequencing of the immunoglobulin or T\u2010cell receptor genes. Tumour clonotypes were identified in 87% of patients with adequate tumour samples. Sixteen of 19 (84%) patients with disease progression after <jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content> had detectable ct<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> prior to progression at a median of 3\u00b77\u00a0months prior to relapse\/progression. Patients with detectable ct<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> 3\u00a0months after <jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content> had inferior progression\u2010free survival (<jats:styled-content style=\"fixed-case\">PFS<\/jats:styled-content>) (2\u2010year <jats:styled-content style=\"fixed-case\">PFS<\/jats:styled-content> 58% vs. 84% in ct<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010negative patients, <jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b7033). In multivariate models, detectable ct<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> was associated with increased risk of progression\/death (Hazard ratio 3\u00b79, <jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b7003) and increased risk of relapse\/progression (Hazard ratio 10\u00b78, <jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b70006). Detectable ct<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> is associated with an increased risk of relapse\/progression, but further validation studies are necessary to confirm these findings and determine the clinical utility of <jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>\u2010based minimal residual disease monitoring in lymphoma patients after <jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/bjh.14311","type":"journal-article","created":{"date-parts":[[2016,10,13]],"date-time":"2016-10-13T01:10:40Z","timestamp":1476321040000},"page":"841-850","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Next\u2010generation sequencing\u2010based detection of circulating tumour <scp>DNA<\/scp> After allogeneic stem cell transplantation for lymphoma"],"prefix":"10.1111","volume":"175","author":[{"given":"Alex F.","family":"Herrera","sequence":"first","affiliation":[{"name":"Department of Hematology and Hematopoietic Cell Transplantation City of Hope Medical Center Duarte CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haesook T.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Computational Biology Dana\u2010Farber Cancer Institute Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine A.","family":"Kong","sequence":"additional","affiliation":[{"name":"Adaptive Biotechnologies Corp. South San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malek","family":"Faham","sequence":"additional","affiliation":[{"name":"Adaptive Biotechnologies Corp. South San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pathology Brigham and Women's Hospital Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliyah R.","family":"Sohani","sequence":"additional","affiliation":[{"name":"Department of Pathology Massachusetts General Hospital Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin P.","family":"Alyea","sequence":"additional","affiliation":[{"name":"Division of Hematologic Malignancies Dana\u2010Farber Cancer Institute Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria E.","family":"Carlton","sequence":"additional","affiliation":[{"name":"Adaptive Biotechnologies Corp. South San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Bin","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Bone Marrow Transplantation Massachusetts General Hospital Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corey S.","family":"Cutler","sequence":"additional","affiliation":[{"name":"Division of Hematologic Malignancies Dana\u2010Farber Cancer Institute Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent T.","family":"Ho","sequence":"additional","affiliation":[{"name":"Division of Hematologic Malignancies Dana\u2010Farber Cancer Institute Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Koreth","sequence":"additional","affiliation":[{"name":"Division of Hematologic Malignancies Dana\u2010Farber Cancer Institute Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chitra","family":"Kotwaliwale","sequence":"additional","affiliation":[{"name":"Adaptive Biotechnologies Corp. 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