{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T20:05:51Z","timestamp":1777320351041,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,2,7]],"date-time":"2017-02-07T00:00:00Z","timestamp":1486425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Allogeneic haematopoietic stem cell transplantation is curative for severe aplastic anaemia (<jats:styled-content style=\"fixed-case\">SAA<\/jats:styled-content>) unresponsive to immunosuppressive therapy. To reduce chronic graft\u2010<jats:italic>versus<\/jats:italic>\u2010host disease (<jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content>), which occurs more frequently after peripheral blood stem cell (<jats:styled-content style=\"fixed-case\">PBSC<\/jats:styled-content>) transplantation compared to bone\u2010marrow transplantation (<jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content>), and to prevent graft rejection, we developed a novel partial T\u2010cell depleted transplant that infuses high numbers of granulocyte colony\u2010stimulating factor\u2010mobilized <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34<jats:sup>+<\/jats:sup> selected <jats:styled-content style=\"fixed-case\">PBSC<\/jats:styled-content>s combined with a <jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content>\u2010equivalent dose of non\u2010mobilized donor T\u2010cells. Fifteen patients with refractory <jats:styled-content style=\"fixed-case\">SAA<\/jats:styled-content> received cyclophosphamide, anti\u2010thymocyte globulin and fludarabine conditioning, and were transplanted with a median 8\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup> <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34<jats:sup>+<\/jats:sup>\u00a0cells\/kg and 2\u00a0\u00d7\u00a010<jats:sup>7<\/jats:sup> non\u2010mobilized <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3<jats:sup>+<\/jats:sup> T\u2010cells\/kg from human leucocyte antigen\u2010matched sibling donors. All achieved sustained engraftment with only two developing acute and two developing chronic <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content>. With a 3\u00b75\u2010year median follow\u2010up, 86% of patients survived and were transfusion\u2010independent. When compared to a retrospective cohort of 56 bone\u2010marrow failure patients that received the identical transplant preparative regimen and <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content> prophylaxis with the exception that the allograft contained unmanipulated <jats:styled-content style=\"fixed-case\">PBSC<\/jats:styled-content>s, partial T\u2010cell depleted transplant recipients had delayed donor T\u2010cell chimerism and relative reduction of 75% in the incidence of acute grade <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">IV GVHD<\/jats:styled-content> (13% vs. 52%; <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0\u00b7010) and of 82% in chronic <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content> (13% vs. 72%; <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0\u00b70004). In multivariate analysis, partial T\u2010cell depleted transplants remained significantly associated with a reduced risk of <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content>. In conclusion, for patients with refractory <jats:styled-content style=\"fixed-case\">SAA<\/jats:styled-content>, this novel transplant strategy achieves excellent engraftment and survival when compared to unmanipulated <jats:styled-content style=\"fixed-case\">PBSC<\/jats:styled-content> transplants and dramatically reduces the incidence of both acute and chronic <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/bjh.14448","type":"journal-article","created":{"date-parts":[[2017,2,7]],"date-time":"2017-02-07T10:36:45Z","timestamp":1486463805000},"page":"950-960","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Allogeneic transplantation using <scp>CD<\/scp>34<sup>+<\/sup> selected peripheral blood progenitor cells combined with non\u2010mobilized donor T\u00a0cells for refractory severe aplastic anaemia"],"prefix":"10.1111","volume":"176","author":[{"given":"Enkhtsetseg","family":"Purev","sequence":"first","affiliation":[{"name":"Hematology Branch National Heart, Lung and Blood Institute National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Tian","sequence":"additional","affiliation":[{"name":"Office of Biostatistics Research National Heart, Lung and Blood Institute National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georg","family":"Aue","sequence":"additional","affiliation":[{"name":"Hematology Branch National Heart, Lung and Blood Institute National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy","family":"Pantin","sequence":"additional","affiliation":[{"name":"Hematology and Oncology Department of Medicine Augusta University Augusta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phuong","family":"Vo","sequence":"additional","affiliation":[{"name":"Hematology Branch National Heart, Lung and Blood Institute National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reem","family":"Shalabi","sequence":"additional","affiliation":[{"name":"Clinical Center Pharmacy Department National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert N.","family":"Reger","sequence":"additional","affiliation":[{"name":"Hematology Branch National Heart, Lung and Blood Institute National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Cook","sequence":"additional","affiliation":[{"name":"Hematology Branch National Heart, Lung and Blood Institute National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catalina","family":"Ramos","sequence":"additional","affiliation":[{"name":"Hematology Branch National Heart, Lung and Blood Institute National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Cho","sequence":"additional","affiliation":[{"name":"Hematology Branch National Heart, Lung and Blood Institute National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tat'yana","family":"Worthy","sequence":"additional","affiliation":[{"name":"Hematology Branch National Heart, Lung and Blood Institute National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanh","family":"Khuu","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Stroncek","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neal S.","family":"Young","sequence":"additional","affiliation":[{"name":"Hematology Branch National Heart, Lung and Blood Institute National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard W.","family":"Childs","sequence":"additional","affiliation":[{"name":"Hematology Branch National Heart, Lung and Blood Institute National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1203517"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdt558"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2014.08.004"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0037-1963(00)90031-3"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.054841"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2014.115345"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-03-563817"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-04-1170"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V73.2.606.606"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2005.04.008"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.9.3234"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2010.10.029"},{"key":"e_1_2_8_14_1","first-page":"347","article-title":"Early reactivation of cytomegalovirus and high risk of interstitial pneumonitis following T\u2010depleted BMT for adults with hematological malignancies","volume":"18","author":"Couriel D.","year":"1996","journal-title":"Bone Marrow Transplantation"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1972.tb00899.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428190903144659"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-7963(98)80085-0"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2005.09.004"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.20467"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0258(19990330)18:6<695::AID-SIM60>3.0.CO;2-O"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05364.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-10-609032"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0037-1963(00)90028-3"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/01621459.1958.10501452"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/b97377"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2006.06352.x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.10075"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2008.03.005"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/00062752-199811000-00015"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-12-327536"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2012.251"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.006916"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2008.390"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23526"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2014.05.012"},{"key":"e_1_2_8_36_1","unstructured":"Pasquini M.&Zhu X.(2015)Current uses and outcomes of hematopoietic stem cell transplantation: 2015 CIBMTR Summary Slides. Available at:https:\/\/www.cibmtr.org\/ReferenceCenter\/SlidesReports\/SummarySlides\/pages\/index.aspx"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-11-319509"},{"key":"e_1_2_8_38_1","first-page":"825","article-title":"1994 Consensus Conference on Acute GVHD Grading","volume":"15","author":"Przepiorka D.","year":"1995","journal-title":"Bone Marrow Transplantation"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2008.262"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1985.tb01158.x"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-12-274019"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1103975"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-03-081596"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2009.80"},{"key":"e_1_2_8_45_1","doi-asserted-by":"crossref","first-page":"2269","DOI":"10.1182\/blood.V95.7.2269","article-title":"Pharmacologic doses of granulocyte colony\u2010stimulating factor affect cytokine production by lymphocytes in\u00a0vitro and in\u00a0vivo","volume":"95","author":"Sloand E.M.","year":"2000","journal-title":"Blood"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2006.06019.x"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1983.00573.x"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-02-268466"},{"key":"e_1_2_8_49_1","first-page":"LBA\u20102","article-title":"Eltrombopag added to standard immunosuppression for aplastic anemia accelerates count recovery and increases response rates","volume":"126","author":"Townsley D.M.","year":"2015","journal-title":"Blood (ASH Annual Meeting Abstracts)"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.2.383"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.27761"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbjh.14448","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.14448","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T20:44:08Z","timestamp":1696279448000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.14448"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,7]]},"references-count":50,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1111\/bjh.14448"],"URL":"https:\/\/doi.org\/10.1111\/bjh.14448","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,7]]},"assertion":[{"value":"2016-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-09-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}