{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T22:10:50Z","timestamp":1789855850619,"version":"4.0.1"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,4,17]],"date-time":"2017-04-17T00:00:00Z","timestamp":1492387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","award":["5\/4\/150\/5"],"award-info":[{"award-number":["5\/4\/150\/5"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","award":["7.4578.14 T\u00e9l\u00e9vie"],"award-info":[{"award-number":["7.4578.14 T\u00e9l\u00e9vie"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Reimplantation of cryopreserved ovarian tissue (\n                    <jats:styled-content style=\"fixed-case\">OT<\/jats:styled-content>\n                    ) can successfully restore ovarian function in young cancer patients after gonadotoxic treatment. However, for patients with leukaemia, there is a risk of malignant cell transmission. Our objective was to evaluate minimal disseminated disease in\n                    <jats:styled-content style=\"fixed-case\">OT<\/jats:styled-content>\n                    from leukaemia patients and test a follicle isolation technique to obtain disease\u2010free follicle suspensions. Cryopreserved\n                    <jats:styled-content style=\"fixed-case\">OT<\/jats:styled-content>\n                    from 12 leukaemia patients was thawed and analysed by histology and long\u2010term xenografting in immunosuppressed mice. In 10 patients, follicles were isolated from\n                    <jats:styled-content style=\"fixed-case\">OT<\/jats:styled-content>\n                    , and polymerase chain reaction (\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    ) was performed on tissue, digested ovarian suspensions and isolated follicle suspensions to investigate leukaemic cell presence. Mean patient age was 17\u00b71\u00a0years. An average of 3\u00b72 follicles were isolated per mm\u00b2 of cortex. Xenografting of\n                    <jats:styled-content style=\"fixed-case\">OT<\/jats:styled-content>\n                    induced leukaemic masses in 2\/12 mice.\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    identified leukaemic cell presence in 66% of\n                    <jats:styled-content style=\"fixed-case\">OT<\/jats:styled-content>\n                    . Malignant cells were also detected in digested ovarian suspensions. However, none of the follicle samples (&gt;2300 follicles tested) showed any malignant cell presence after washing. This study demonstrates that it is possible to recover large numbers of viable follicles from cryopreserved\n                    <jats:styled-content style=\"fixed-case\">OT<\/jats:styled-content>\n                    of leukaemia patients. All isolated and washed follicle suspensions tested negative for leukaemic cells, giving leukaemia patients genuine hope of fertility restoration.\n                  <\/jats:p>","DOI":"10.1111\/bjh.14657","type":"journal-article","created":{"date-parts":[[2017,4,17]],"date-time":"2017-04-17T05:08:41Z","timestamp":1492405721000},"page":"231-239","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Eliminating malignant cells from cryopreserved ovarian tissue is possible in leukaemia patients"],"prefix":"10.1111","volume":"178","author":[{"given":"Michelle","family":"Soares","sequence":"first","affiliation":[{"name":"P\u00f4le de Recherche en Gyn\u00e9cologie Institut de Recherche Exp\u00e9rimentale et Clinique Universit\u00e9 Catholique de Louvain Brussels Belgium"},{"name":"D\u00e9partement de Gyn\u00e9cologie Cliniques Universitaires St. Luc Brussels 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