{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:46:59Z","timestamp":1791362819652,"version":"4.3.1"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,3]],"date-time":"2017-12-03T00:00:00Z","timestamp":1512259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Poor graft function (<jats:styled-content style=\"fixed-case\">PGF<\/jats:styled-content>) is a severe complication of haematopoietic stem cell transplantation (<jats:styled-content style=\"fixed-case\">HSCT<\/jats:styled-content>) and administration of donor stem cell boosts (<jats:styled-content style=\"fixed-case\">SCB<\/jats:styled-content>s) represents a therapeutic option. We report 50 paediatric patients with <jats:styled-content style=\"fixed-case\">PGF<\/jats:styled-content> who received 61 boosts with <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34<jats:sup>+<\/jats:sup> selected peripheral blood stem cells (<jats:styled-content style=\"fixed-case\">PBSC<\/jats:styled-content>) after transplantation from matched unrelated (<jats:italic>n<\/jats:italic>\u00a0=\u00a025) or mismatched related (<jats:italic>n<\/jats:italic>\u00a0=\u00a025) donors. Within 8\u00a0weeks, a significant increase in median neutrophil counts (0\u00b76 vs. 1\u00b7516\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup>\/l, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b705) and a decrease in red blood cell and platelet transfusion requirement (median frequencies 1 and 7 vs. 0, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b70001 and &lt;0\u00b7001), were observed, and 78\u00b78% of patients resolved one or two of their cytopenias. 36\u00b75% had a complete haematological response. Median lymphocyte counts for <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3<jats:sup>+<\/jats:sup>, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3<jats:sup>+<\/jats:sup><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup>, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>19<jats:sup>+<\/jats:sup> and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>56<jats:sup>+<\/jats:sup> increased 8\u00b73\u2010, 14\u00b72\u2010, 22.\u2010 and 1\u00b76\u2010fold. The rate of <jats:italic>de novo<\/jats:italic> acute graft\u2010versus\u2010host disease (Gv<jats:styled-content style=\"fixed-case\">HD<\/jats:styled-content>) grade I\u2013III was only 6% and resolved completely. No Gv<jats:styled-content style=\"fixed-case\">HD<\/jats:styled-content> grade <jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content> or chronic Gv<jats:styled-content style=\"fixed-case\">HD<\/jats:styled-content> occurred. Patients who responded to <jats:styled-content style=\"fixed-case\">SCB<\/jats:styled-content> displayed a trend toward better overall survival (<jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content>) (<jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b707). Thus, administration of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34<jats:sup>+<\/jats:sup> selected <jats:styled-content style=\"fixed-case\">SCB<\/jats:styled-content>s from alternative donors is safe and effective. Further studies are warranted to clarify the impact on immune reconstitution and survival.<\/jats:p>","DOI":"10.1111\/bjh.15012","type":"journal-article","created":{"date-parts":[[2017,12,5]],"date-time":"2017-12-05T10:24:27Z","timestamp":1512469467000},"page":"90-99","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["CD34<sup>+<\/sup> selected stem cell boosts can improve poor graft function after paediatric allogeneic stem cell transplantation"],"prefix":"10.1111","volume":"180","author":[{"given":"Chiara","family":"Mainardi","sequence":"first","affiliation":[{"name":"Department of Paediatric Oncology Children's University Hospital University of Padova Padova Italy"},{"name":"Department of Paediatric Haematology\/Oncology Children's University Hospital T\u00fcbingen 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