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We therefore determined the cell cycle status of leukaemic cells by immunophenotypic analysis of bone marrow trephine biopsies from 181 patients with acute myeloid leukaemia (<jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content>) and correlated the results with biological features and clinical outcome. There was considerable heterogeneity between patients. The presenting white cell count significantly correlated with the proportion of non\u2010quiescent cells (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0\u00b70001), of cycling cells beyond G<jats:sub>1<\/jats:sub> (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0\u00b70001) and the speed of cycling (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0\u00b70001). Profiles in acute promyelocytic leukaemia (<jats:styled-content style=\"fixed-case\">APL<\/jats:styled-content>) differed from non\u2010<jats:styled-content style=\"fixed-case\">APL<\/jats:styled-content> and were consistent with more differentiated cells with reduced proliferative potential, but no significant differences were observed between non\u2010<jats:styled-content style=\"fixed-case\">APL<\/jats:styled-content> cytogenetic risk groups. <jats:italic><jats:styled-content style=\"fixed-case\">NPM<\/jats:styled-content>1<\/jats:italic> mutations but not <jats:italic><jats:styled-content style=\"fixed-case\">FLT<\/jats:styled-content>3<\/jats:italic> internal tandem duplication (<jats:italic><jats:styled-content style=\"fixed-case\">FLT<\/jats:styled-content>3<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:sup>ITD<\/jats:sup><\/jats:styled-content>) were significantly associated with a higher proportion of cells beyond G<jats:sub>1<\/jats:sub> (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0\u00b7002) and faster speed of cycling (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0\u00b7003). Resistance to standard cytosine arabinoside and daunorubicin induction chemotherapy was significantly related to a slower speed of cycling (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0\u00b70002), as was a higher relapse rate (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0\u00b705), but not with the proportion of non\u2010quiescent cells or actively cycling cells. These results show a link between the cycling speed of <jats:styled-content style=\"fixed-case\">AML<\/jats:styled-content> cells and the response to chemotherapy, and help to identify a group with a very poor prognosis.<\/jats:p>","DOI":"10.1111\/bjh.15211","type":"journal-article","created":{"date-parts":[[2018,4,20]],"date-time":"2018-04-20T11:47:54Z","timestamp":1524224874000},"page":"486-494","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Immunophenotypic analysis of cell cycle status in acute myeloid leukaemia: relationship to cytogenetics, genotype and clinical outcome"],"prefix":"10.1111","volume":"181","author":[{"given":"Rob S.","family":"Sellar","sequence":"first","affiliation":[{"name":"Department of Haematology UCL Cancer Institute London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0103-1787","authenticated-orcid":false,"given":"Rosemary 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