{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:45:38Z","timestamp":1787013938011,"version":"build-2736575974"},"reference-count":64,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,6,19]],"date-time":"2019-06-19T00:00:00Z","timestamp":1560902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2011\/50358-3"],"award-info":[{"award-number":["2011\/50358-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Beta\u2010thalassaemia (BT) is classified according to blood transfusion requirement as minor (BTMi), intermedia (BTI) and major (BTM). BTM is the most severe form, requiring regular transfusions while transfusion need is only occasional in BTI. Differential gene expression between patients has not been assessed so far. Here, we evaluated the global gene expression profiles during differentiation of human erythroid cells of two patients carrying the same mutation [\n                    <jats:italic>CD39<\/jats:italic>\n                    ,\u00a0(C \u2192 T)], though displaying different phenotypes (BTI and BTM). Considering the role of reactive oxygen species (ROS) in the pathophysiology of thalassaemia, we focused on differentially expressed genes involved in metabolic pathways triggered by ROS, such as inflammation and apoptosis, and, from these, we selected the Apurinic\/Apyrimidinic Endodeoxyribonuclease 1 (\n                    <jats:italic>APEX1<\/jats:italic>\n                    ) and High Mobility Group Box1 (\n                    <jats:italic>HMGB1<\/jats:italic>\n                    ) genes, whose role in BT is not well established. An in\u2010depth expression analysis of transcriptional and protein levels in patients carrying a range of mutations associated with BT phenotypes indicated that APEX1 was increased in both BTI and BTM. Furthermore, higher amounts of HMGB1 was found in the plasma of BTI patients. Our findings suggest that these proteins have important roles in BT and could represent new targets for further studies aiming to improve the management of the disease.\n                  <\/jats:p>","DOI":"10.1111\/bjh.16062","type":"journal-article","created":{"date-parts":[[2019,6,19]],"date-time":"2019-06-19T21:30:20Z","timestamp":1560979820000},"page":"608-619","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Global gene expression reveals an increase of HMGB1 and APEX1 proteins and their involvement in oxidative stress, apoptosis and inflammation pathways among beta\u2010thalassaemia intermedia and major phenotypes"],"prefix":"10.1111","volume":"186","author":[{"given":"Jo\u00e3o Pedro","family":"Maia de Oliveira da Silva","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade Universidade Federal de S\u00e3o Carlos S\u00e3o Carlos S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Fl\u00e1via","family":"Brugnerotto","sequence":"additional","affiliation":[{"name":"Centro de Hematologia e Hemoterapia Universidade Estadual de Campinas Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"S. Romanello","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade Universidade Federal de S\u00e3o Carlos S\u00e3o Carlos S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"K. L. Teixeira","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade Universidade Federal de S\u00e3o Carlos S\u00e3o Carlos S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Lanaro","sequence":"additional","affiliation":[{"name":"Centro de Hematologia e Hemoterapia Universidade Estadual de Campinas Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"S. Duarte","sequence":"additional","affiliation":[{"name":"Centro de Hematologia e Hemoterapia Universidade Estadual de Campinas Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"G. L. Costa","sequence":"additional","affiliation":[{"name":"Centro Nacional de Processamento de Alto Desempenho em S\u00e3o Paulo. CENAPAD\u2010SP Campinas S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aderson","family":"da Silva Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o de Hematologia e Hemoterapia de Pernambuco. HEMOPE Recife Pernambuco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Andr\u00e9","family":"C. Bezerra","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica Universidade Federal de Pernambuco Recife Pernambuco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"de Farias Domingos","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica Universidade Federal de Pernambuco Recife Pernambuco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego A.","family":"Pereira Martins","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica Universidade Federal de Pernambuco Recife Pernambuco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iran","family":"Malavazi","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica e Evolu\u00e7\u00e3o, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade Universidade Federal de S\u00e3o Carlos S\u00e3o Carlos S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"F. 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