{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T17:49:59Z","timestamp":1762624199300,"version":"3.40.5"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,8,5]],"date-time":"2019-08-05T00:00:00Z","timestamp":1564963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Since 2000, we have investigated 67 consecutive patients with stage I\/II follicular lymphoma (FL) for the presence of <jats:italic>BCL2\/IGH<\/jats:italic> rearrangements by polymerase chain reaction (PCR), real time quantitative PCR (RQ\u2010PCR) and digital droplet PCR (ddPCR). All patients were treated with involved\u2010field radiotherapy (IF\u2010RT) (24\u201330\u00a0Gy). From 2005, patients with minimal residual disease (MRD) after IF\u2010RT received rituximab (R) (375\u00a0mg\/m<jats:sup>2<\/jats:sup>, 4 weekly administrations). The median follow\u2010up is 82\u00a0months (17\u2013196). At diagnosis, 72% of patients were <jats:italic>BCL2\/IGH<\/jats:italic>+. Progression\u2010free survival (PFS) was significantly better in patients with undetectable\/low levels (&lt;10<jats:sup>\u22125<\/jats:sup>) of circulating <jats:italic>BCL2\/IGH<\/jats:italic>+ cells at diagnosis and in those who were persistently MRD\u2212 during follow\u2010up (<jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b70038). IF\u2010RT induced an MRD\u2212 status in 50% of cases; 16\/19 (84%) MRD+ patients after IF\u2010RT became MRD\u2212 after R treatment. A significantly longer PFS was observed in MRD+ patients treated with R compared to untreated MRD+ patients (<jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b7049). In early stage FL, both circulating levels of <jats:italic>BCL2\/IGH<\/jats:italic>+ cells at diagnosis and MRD status during follow\u2010up bear prognostic implications. Standard IF\u2010RT fails to induce an MRD\u2010negative status in half of patients. Most patients become MRD\u2212 following treatment with R and this is associated with a significantly better PFS.<\/jats:p>","DOI":"10.1111\/bjh.16125","type":"journal-article","created":{"date-parts":[[2019,8,6]],"date-time":"2019-08-06T00:11:17Z","timestamp":1565050277000},"page":"249-258","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Minimal residual disease monitoring in early stage follicular lymphoma can predict prognosis and drive treatment with rituximab after radiotherapy"],"prefix":"10.1111","volume":"188","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7429-8004","authenticated-orcid":false,"given":"Alessandro","family":"Pulsoni","sequence":"first","affiliation":[{"name":"Haematology, Department of Translational and Precision Medicine Sapienza University Rome 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