{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T21:42:41Z","timestamp":1757454161027,"version":"3.40.5"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,2,9]],"date-time":"2020-02-09T00:00:00Z","timestamp":1581206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009647","name":"Ministry of Health","doi-asserted-by":"publisher","award":["17\u201030397A"],"award-info":[{"award-number":["17\u201030397A"]}],"id":[{"id":"10.13039\/100009647","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The occurrence of mutations in the <jats:italic>BCR\u2010ABL1<\/jats:italic> kinase domain (KD) can lead to treatment resistance in chronic myeloid leukaemia patients. Nowadays, next\u2010generation sequencing (NGS) is an alternative method for the detection of kinase domain mutations, compared to routinely used Sanger sequencing, providing a higher sensitivity of mutation detection. However, in the protocols established so far multiple rounds of amplification limit reliable mutation detection to approximately 5% variant allele frequency. Here, we present a simplified, one\u2010round amplification NGS protocol for the Illumina platform, which offers a robust early detection of <jats:italic>BCR\u2010ABL1<\/jats:italic> KD mutations with a reliable detection limit of 3% variant allele frequency.<\/jats:p>","DOI":"10.1111\/bjh.16382","type":"journal-article","created":{"date-parts":[[2020,2,10]],"date-time":"2020-02-10T05:58:15Z","timestamp":1581314295000},"page":"469-474","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Novel Illumina\u2010based next generation sequencing approach with one\u2010round amplification provides early and reliable detection of BCR\u2010ABL1 kinase domain mutations in chronic myeloid leukemia"],"prefix":"10.1111","volume":"189","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0708-3198","authenticated-orcid":false,"given":"Marianna","family":"Romzova","sequence":"first","affiliation":[{"name":"Department of Molecular 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