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It is known that <jats:italic>Bcl\u2010x<\/jats:italic> splicing is deregulated and is involved in multiple malignant cancer initiation and chemotherapy resistance, including CML. The aim of the present study was to correct the abnormal splicing of <jats:italic>Bcl\u2010x<\/jats:italic> in CML and investigate the subsequent malignant phenotype changes, especially response to IM. The aberrant <jats:italic>Bcl\u2010x<\/jats:italic> splicing in CML cells was effectively restored using vivo\u2010Morpholino Antisense Oligomer (vMO). CCK\u20108 cell viability assay and flow cytometry showed that restoring of <jats:italic>Bcl\u2010x<\/jats:italic> splicing increases IM\u2010induced growth inhibition and apoptosis of K562 cells. Moreover, a more significant similar phenomenon was observed in imatinib\u2010resistant CML cell lines K562\/G01. Finally, establishment of CML xenograft model had also proved that correcting <jats:italic>Bcl\u2010x<\/jats:italic> splicing <jats:italic>in vivo<\/jats:italic> can also enhance the anti\u2010tumor effect of IM. Our findings suggest that vMO co\u2010operating with IM can effectively increase the sensitivity of CML cells to IM both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>, and <jats:italic>Bcl\u2010x<\/jats:italic> splicing could become good candidates for chemotherapy\u2010sensitized target in IM\u2010resistant CML.<\/jats:p>","DOI":"10.1111\/bjh.16472","type":"journal-article","created":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T08:09:43Z","timestamp":1584605383000},"page":"1141-1150","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Correction of <i>Bcl<\/i><i>\u2010x<\/i> splicing improves responses to imatinib in chronic myeloid leukaemia cells and mouse models"],"prefix":"10.1111","volume":"189","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2172-4708","authenticated-orcid":false,"given":"Jing","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of 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