{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T16:09:53Z","timestamp":1790525393767,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T00:00:00Z","timestamp":1586217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Association Recherche et Transfusion"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is the consequence of platelet destruction by maternal alloantibodies against fetal human platelet antigens (HPA). This may result in intracranial haemorrhages (ICH) or even fetal death. Currently, fetal HPA genotyping is performed using invasive procedures. Here, we carried out a proof\u2010of\u2010concept study for non\u2010invasive prenatal diagnosis of fetal platelet genotyping in four HPA systems (HPA\u20101, \u20103, \u20105 and\u201015) by droplet digital polymerase chain reaction (ddPCR) using cell\u2010free DNA extracts from the plasma of 47 pregnant women with suspected, or history of, FNAIT. Results showed that 74% (35\/47) of pregnant women presented incompatibility in at least one HPA system, and 38% (18\/47) of cases presented HPA\u20101 incompatibility, including nine women with multiple incompatibilities. ICH occurred in one case of profound fetal thrombocytopenia with HPA\u201015 incompatibility, confirming the need for non\u2010invasive prenatal genotyping in systems other than HPA\u20101. Fetal HPA genotypes predicted by ddPCR were confirmed in all FNAIT cases after amniocentesis or delivery. Fetal HPA genotyping on maternal plasma based on ddPCR is a fast, safe and reliable non\u2010invasive method. This technique will be useful for the early identification of pregnancies at high risk of FNAIT requiring antenatal management to minimize the risk of fetal\/neonatal haemorrhage.<\/jats:p>","DOI":"10.1111\/bjh.16593","type":"journal-article","created":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T05:13:03Z","timestamp":1586322783000},"page":"787-798","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["A new efficient\u00a0tool for non\u2010invasive diagnosis of fetomaternal platelet antigen incompatibility"],"prefix":"10.1111","volume":"190","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6361-8885","authenticated-orcid":false,"given":"Yasmine","family":"Ouzegdouh Mammasse","sequence":"first","affiliation":[{"name":"Institut National de la Transfusion Sanguine (INTS) D\u00e9partement d\u2019Immunologie Plaquettaire Paris 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1287 Gustave Roussy Villejuif France"},{"name":"Universit\u00e9 Paris\u2010Saclay UMR1287 Gustave Roussy Villejuif France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2082-6278","authenticated-orcid":false,"given":"Rachel","family":"Petermann","sequence":"additional","affiliation":[{"name":"Institut National de la Transfusion Sanguine (INTS) D\u00e9partement d\u2019Immunologie Plaquettaire Paris France"},{"name":"Centre de Recherche des Cordeliers UMRS 1138 INSERM Sorbonne Universit\u00e9 Universit\u00e9 de Paris, Equipe ETRES (Ethics, Research, Translations) Paris 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