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Recently, it has been demonstrated that it is possible to detect molecular alterations analysing cell\u2010free DNA (cfDNA) in plasma samples, which is known as liquid biopsy. We have assessed the molecular profile of a cohort of 107 MPN patients [33 polycythaemia vera (PV), 56 essential thrombocythaemia (ET), 14 primary myelofibrosis (PMF) and 4 unclassifiable MPN] by next\u2010generation sequencing (NGS) using cfDNA and paired granulocyte DNA. A high concentration of cfDNA in plasma was observed in patients with high molecular complexity, in <jats:italic>MPL<\/jats:italic>\u2010mutated cases, and in PMF patients. Targeted sequencing of cfDNA showed a comparable mutational profile (100% accuracy) to the one obtained in granulocytic DNA and a strong correlation was observed between the variant allele frequency (VAF) of gene mutations in both DNA sources. The median VAF detected in cfDNA (29\u00b70%; range: 0\u00b795\u201391\u00b773%) was significantly higher than the VAF detected in granulocytes (median 25\u00b72%; range: 0\u00b710\u201395\u00b75%), especially for <jats:italic>MPL<\/jats:italic> mutations (44\u00b73% vs. 22\u00b75%). In conclusion, our data support the use of cfDNA as a fast, sensitive and accurate strategy for performing molecular characterisation of MPN patients.<\/jats:p>","DOI":"10.1111\/bjh.17087","type":"journal-article","created":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T11:56:33Z","timestamp":1600430193000},"page":"300-309","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Circulating cell\u2010free DNA improves the molecular characterisation of Ph\u2010negative myeloproliferative neoplasms"],"prefix":"10.1111","volume":"192","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8185-9786","authenticated-orcid":false,"given":"Nieves","family":"Garcia\u2010Gisbert","sequence":"first","affiliation":[{"name":"Group of Applied Clinical Research in Haematology Cancer Research Program\u2010IMIM (Hospital del Mar Medical Research Institute Barcelona Spain"},{"name":"Pompeu Fabra University Barcelona 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