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In the present study, a robust flow cytometric definition for MPO positivity was established based on the standardised EuroFlow protocols, the standardised Acute Leukaemia Orientation Tube and 1734 multicentre AL cases (with confirmed assay stability). The best diagnostic performance was achieved by defining MPO positivity as \u226520% of the AL cells exceeding a lymphocyte\u2010based threshold. The methodology employed should be applicable to any form of standardised flow cytometry.<\/jats:p>","DOI":"10.1111\/bjh.17210","type":"journal-article","created":{"date-parts":[[2020,11,8]],"date-time":"2020-11-08T05:45:54Z","timestamp":1604814354000},"page":"922-927","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia"],"prefix":"10.1111","volume":"193","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5094-7700","authenticated-orcid":false,"given":"Anne E.","family":"Bras","sequence":"first","affiliation":[{"name":"Laboratory Medical Immunology (LMI) Department of Immunology Erasmus MC University Medical Center Rotterdam Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zgjim","family":"Osmani","sequence":"additional","affiliation":[{"name":"Laboratory Medical Immunology (LMI) Department of Immunology Erasmus MC University Medical Center Rotterdam Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie","family":"de Haas","sequence":"additional","affiliation":[{"name":"Dutch Childhood Oncology Group Utrecht the Netherlands"},{"name":"Princess M\u00e1xima Center for Pediatric Oncology Utrecht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojca","family":"Jongen\u2010Lavrencic","sequence":"additional","affiliation":[{"name":"Department of Hematology Erasmus MC University Medical Center Rotterdam Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen G.","family":"te Marvelde","sequence":"additional","affiliation":[{"name":"Laboratory Medical Immunology (LMI) Department of Immunology Erasmus MC University Medical Center Rotterdam Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Michel","family":"Zwaan","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology Utrecht the Netherlands"},{"name":"Department of Pediatric Oncology\/Hematology Erasmus MC University Medical Center Rotterdam Rotterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ester","family":"Mejstrikova","sequence":"additional","affiliation":[{"name":"CLIP\u2014Childhood Leukaemia Investigation Prague Department of Paediatric Haematology and Oncology Second Faculty of Medicine Charles University University Hospital Motol Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Fernandez","sequence":"additional","affiliation":[{"name":"Institute for Laboratory Medicine Kantonsspital Aarau AG Aarau Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Szczepanski","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology, Zabrze Medical University of Silesia (SUM) Katowice Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0007-7230","authenticated-orcid":false,"given":"Alberto","family":"Orfao","sequence":"additional","affiliation":[{"name":"Cancer Research Center (IBMCC\u2010CSIC) Department of Medicine and Cytometry Service University of Salamanca (USAL) Institute of Biomedical Research of Salamanca (IBSAL) Salamanca Spain"},{"name":"Centro de Investigaci\u03ccn Biom\u00e9dicaen Red de C\u00e1ncer Instituto Carlos III Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques J. 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