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Recently, next\u2010generation sequencing (NGS)\u2010based approaches have been developed that afford greater sensitivity and straightforward discrimination between compound and polyclonal mutations. We performed a study to compare the results of SS and NGS in a consecutive cohort of 171 Ph+ ALL patients. At diagnosis, 0\/44 and 3\/44 patients were positive for mutations by SS and NGS respectively. Out of 47 patients with haematologic resistance, 45 had mutations according to both methods, but in 25 patients NGS revealed additional mutations undetectable by SS. Out of 80 patients in complete haematologic response but with <jats:italic>BCR\u2010ABL1<\/jats:italic> \u22650\u00b71%, 28 (35%) and 52 (65%) were positive by SS and NGS respectively. Moreover, in 12 patients positive by SS, NGS detected additional mutations. NGS resolved clonal complexity in 34 patients with multiple mutations at the same or different codons and identified 35 compound mutations. Our study demonstrates that, in Ph+ ALL on TKI therapy, NGS enables more accurate assessment of mutation status both in patients who fail therapy and in patients with minimal residual disease above 0\u00b71%.<\/jats:p>","DOI":"10.1111\/bjh.17301","type":"journal-article","created":{"date-parts":[[2021,1,7]],"date-time":"2021-01-07T08:25:02Z","timestamp":1610007902000},"page":"271-279","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Next\u2010generation sequencing improves <i>BCR\u2010ABL1<\/i> mutation detection in Philadelphia chromosome\u2010positive acute lymphoblastic leukaemia"],"prefix":"10.1111","volume":"193","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4508-0353","authenticated-orcid":false,"given":"Simona","family":"Soverini","sequence":"first","affiliation":[{"name":"Department of Experimental, Diagnostic and Specialty Medicine Institute of Hematology \u201cL. e A. 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