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CAR cells are characterized by several salient features, including much higher expression of CXCL12, stem cell factor (SCF), forkhead box C1 (FOXC1) and early B\u2010cell factor 3 (EBF3), which are essential for HSC maintenance, than other cells. However, the human counterpart of CAR cells has not been fully described. Here, we show the presence of cells expressing much higher CXCL12 than other cells in human adult bone marrow using a flow cytometry\u2010based <jats:italic>in situ<\/jats:italic> technique that enables high\u2010throughput detection of mRNA at single\u2010cell resolution. Most CXCL12<jats:sup>hi<\/jats:sup> cells expressed high levels of SCF, FOXC1 and EBF3 and had the potential to differentiate into adipocytes and osteoblasts. Histologically, the nuclei of CXCL12<jats:sup>hi<\/jats:sup> cells were identified and quantified by EBF3 expression in fixed marrow sections. CXCL12<jats:sup>hi<\/jats:sup> cells sorted from residual bone marrow aspirates of chronic myeloid leukaemia patients expressed reduced levels of CXCL12, SCF, FOXC1 and EBF3 in correlation with increased leukaemic burden. Together, we identified the human counterpart of CAR cells, enabling the evaluation of their alterations in various haematological disorders by flow cytometric and histological analyses.<\/jats:p>","DOI":"10.1111\/bjh.17396","type":"journal-article","created":{"date-parts":[[2021,4,10]],"date-time":"2021-04-10T09:10:49Z","timestamp":1618045849000},"page":"659-668","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":76,"title":["Identification of CXCL12\u2010abundant reticular cells in human adult bone marrow"],"prefix":"10.1111","volume":"193","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2172-2195","authenticated-orcid":false,"given":"Kazunari","family":"Aoki","sequence":"first","affiliation":[{"name":"Laboratory of Stem Cell Biology and Developmental Immunology Immunology Frontier Research Center World Premier International Research Center (WPI) Graduate School of Frontier Biosciences Graduate School of Medicine Osaka 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