{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T01:15:03Z","timestamp":1777598103035,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,5,6]],"date-time":"2021-05-06T00:00:00Z","timestamp":1620259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100018540","name":"Natural Science Foundation of Guangdong Province for Distinguished Young Scholars","doi-asserted-by":"publisher","award":["2019A1515010094"],"award-info":[{"award-number":["2019A1515010094"]}],"id":[{"id":"10.13039\/501100018540","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671585"],"award-info":[{"award-number":["81671585"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["8190011412"],"award-info":[{"award-number":["8190011412"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Sophisticated cross\u2010talk between bone marrow mesenchymal stromal cells (BM MSCs) and haematopoietic\/leukaemic stem cells in patients with myelodysplastic syndromes (MDS) and myeloid leukaemia have been emphasized in previous reports. However, mesenchymal elements in patients with chronic myelomonocytic leukaemia (CMML) were poorly investigated. By utilizing a parallel RNA\u2010sequencing method, we investigated the transcriptional profile and functional defects of primary BM MSCs from patients with CMML for the first time. Within a 24\u2010patient cohort, transcriptional and functional analysis reveals a prominent enrichment of WNT\/\u03b2\u2010catenin signalling and multiple biology processes. Deregulated expression of WNT\/\u03b2\u2010catnin factors <jats:italic>CTNNB1<\/jats:italic>, <jats:italic>CMYC<\/jats:italic>, <jats:italic>LEF1<\/jats:italic>, and <jats:italic>FRZB<\/jats:italic> is associated with impaired proliferation, senescence phenotype, and abnormal secretion in CMML MSCs. The impaired ability to support healthy CD34<jats:sup>+<\/jats:sup> haematopoietic stem and progenitor cells (HSPCs) correlates with activation of WNT\/\u03b2\u2010catenin signalling in CMML MSCs. Furthermore, we observed an association between WNT\/\u03b2\u2010catenin factors and treatment response to hypomethylating agents (HMAs) in a cohort of patients with MDS\/myeloproliferative neoplasms (MPNs). Taken together, our study provides evidence for transcriptional and functional abnormalities in CMML MSCs, and suggests potential prognostic value of evaluating WNT\/\u03b2\u2010catenin signalling in patients with CMML.<\/jats:p>","DOI":"10.1111\/bjh.17425","type":"journal-article","created":{"date-parts":[[2021,5,8]],"date-time":"2021-05-08T16:29:07Z","timestamp":1620491347000},"page":"928-940","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Bone marrow mesenchymal stromal cells in chronic myelomonocytic leukaemia: overactivated WNT\/\u03b2\u2010catenin signalling by parallel RNA sequencing and dysfunctional phenotypes"],"prefix":"10.1111","volume":"193","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9961-2549","authenticated-orcid":false,"given":"Ruohao","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Hematology Guangdong Provincial People\u2019s Hospital Guangdong Academy of Medical Sciences School of Medicine South China University of Technology Guangzhou Guangdong 510080 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Hematology Guangdong Provincial People\u2019s Hospital Guangdong Academy of Medical Sciences School of Medicine South China University of Technology Guangzhou Guangdong 510080 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hematology Guangdong Provincial People\u2019s Hospital Guangdong Academy of Medical Sciences School of Medicine South China University of Technology Guangzhou Guangdong 510080 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengxin","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Hematology Guangdong Provincial People\u2019s Hospital Guangdong Academy of Medical Sciences School of Medicine South China University of Technology Guangzhou Guangdong 510080 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hematology Guangdong Provincial People\u2019s Hospital Guangdong Academy of Medical Sciences School of Medicine South China University of Technology Guangzhou Guangdong 510080 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Hematology Guangdong Provincial People\u2019s Hospital Guangdong Academy of Medical Sciences School of Medicine South China University of Technology Guangzhou Guangdong 510080 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suxia","family":"Geng","sequence":"additional","affiliation":[{"name":"Department of Hematology Guangdong Provincial People\u2019s Hospital Guangdong Academy of Medical Sciences School of Medicine South China University of Technology Guangzhou Guangdong 510080 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peilong","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Hematology Guangdong Provincial People\u2019s Hospital Guangdong Academy of Medical Sciences School of Medicine South China University of Technology Guangzhou Guangdong 510080 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zesheng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Hematology Guangdong Provincial People\u2019s Hospital Guangdong Academy of Medical Sciences School of Medicine South China University of Technology Guangzhou Guangdong 510080 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianyu","family":"Weng","sequence":"additional","affiliation":[{"name":"Department of Hematology Guangdong Provincial People\u2019s Hospital Guangdong Academy of Medical Sciences School of Medicine South China University of Technology Guangzhou Guangdong 510080 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2648-9433","authenticated-orcid":false,"given":"Xin","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Hematology Guangdong Provincial People\u2019s Hospital Guangdong Academy of Medical Sciences School of Medicine South China University of Technology Guangzhou Guangdong 510080 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.25684"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.27.1361"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/5921392"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2018026955"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08851"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12883"},{"issue":"7","key":"e_1_2_8_8_1","first-page":"3462","article-title":"Premature exhaustion of mesenchymal stromal cells from myelodysplastic syndrome patients","volume":"9","author":"Pang Y","year":"2017","journal-title":"Am J Transl Res."},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.03.033"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02872"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20153751"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-08-736454"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.196"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-02-412890"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-019-0490-0"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-643544"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240600855905"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-08-304287"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.392"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-18541-1"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20497"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M502210200"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-228825"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.207217"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14131"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05862"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-08-452938"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-10-4149"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.34.4226"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-05-352039"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2016.1138295"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2015.10.005"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2016.08.021"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2013.193"},{"issue":"7","key":"e_1_2_8_35_1","first-page":"818","article-title":"Role of HIF1A in the development of myelodysplastic syndromes","volume":"60","author":"Hayashi Y","year":"2019","journal-title":"[Rinsho ketsueki] The Japanese journal of Clinical Hematology"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-03-838946"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-17-1203"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2008.12.008"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.198093"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071875"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2018.202846"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-018-0526-3"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-019-1187-z"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2018022053"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0310-y"}],"updated-by":[{"DOI":"10.1111\/bjh.18756","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T00:00:00Z","timestamp":1679011200000}}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.17425","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bjh.17425","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.17425","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T09:20:37Z","timestamp":1693300837000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.17425"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,6]]},"references-count":44,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1111\/bjh.17425"],"URL":"https:\/\/doi.org\/10.1111\/bjh.17425","archive":["Portico"],"relation":{"erratum":[{"id-type":"doi","id":"10.1111\/bjh.18756","asserted-by":"object"}]},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,6]]},"assertion":[{"value":"2020-11-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}