{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T21:34:01Z","timestamp":1776720841584,"version":"3.51.2"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,7,27]],"date-time":"2021-07-27T00:00:00Z","timestamp":1627344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000289","name":"Cancer Research UK","doi-asserted-by":"publisher","award":["C355\/A26819"],"award-info":[{"award-number":["C355\/A26819"]}],"id":[{"id":"10.13039\/501100000289","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006301","name":"Center for International Business Education and Research, University of Illinois at Urbana-Champaign","doi-asserted-by":"publisher","award":["CB16\/12\/00233"],"award-info":[{"award-number":["CB16\/12\/00233"]}],"id":[{"id":"10.13039\/100006301","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The Hodgkin lymphoma (HL) genomic landscape is hardly known due to the scarcity of tumour cells in the tissue. Liquid biopsy employing circulating tumour DNA (ctDNA) can emerge as an alternative tool for non\u2010invasive genotyping. By using a custom next generation sequencing (NGS) panel in combination with unique molecule identifiers, we aimed to identify somatic variants in the ctDNA of 60 HL at diagnosis. A total of 277 variants were detected in 36 of the 49 samples (73\u00b75%) with a good quality ctDNA sample. The median number of variants detected per patient was five (range 1\u201323) with a median variant allele frequency of 4\u00b72% (0\u00b784\u201328%). Genotyping revealed somatic variants in the following genes: <jats:italic>SOCS1 (<\/jats:italic>28%), <jats:italic>IGLL5<\/jats:italic> (26%), <jats:italic>TNFAIP3 (23%), GNA13<\/jats:italic> (23%), <jats:italic>STAT6<\/jats:italic> (21%) and <jats:italic>B2M (<\/jats:italic>19%). Moreover, several poor prognosis features (high LDH, low serum albumin, B\u2010symptoms, IPI \u2265 3 or at an advanced stage) were related to significantly higher amounts of ctDNA. Variant detection in ctDNA by NGS is a feasible approach to depict the genetic features of HL patients at diagnosis. Our data favour the implementation of liquid biopsy genotyping for the routine evaluation of HL patients.<\/jats:p>","DOI":"10.1111\/bjh.17719","type":"journal-article","created":{"date-parts":[[2021,7,27]],"date-time":"2021-07-27T07:35:29Z","timestamp":1627371329000},"page":"542-551","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Liquid biopsy: a non\u2010invasive approach for Hodgkin lymphoma genotyping"],"prefix":"10.1111","volume":"195","author":[{"given":"Miguel","family":"Alcoceba","sequence":"first","affiliation":[{"name":"Department of Hematology University Hospital of Salamanca (HUS\/IBSAL) CIBERONC and Cancer Research Institute of Salamanca\u2010IBMCC (USAL\u2010CSIC) Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Garc\u00eda\u2010\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Department of Hematology University Hospital of Salamanca (HUS\/IBSAL) CIBERONC and Cancer Research Institute of Salamanca\u2010IBMCC (USAL\u2010CSIC) Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Carmen","family":"Chill\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Hematology University Hospital of Salamanca (HUS\/IBSAL) CIBERONC and Cancer Research Institute of Salamanca\u2010IBMCC (USAL\u2010CSIC) Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5543-5154","authenticated-orcid":false,"given":"Cristina","family":"Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Department of Hematology University Hospital of Salamanca (HUS\/IBSAL) CIBERONC and Cancer Research Institute of Salamanca\u2010IBMCC (USAL\u2010CSIC) Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Medina","sequence":"additional","affiliation":[{"name":"Department of Hematology University Hospital of Salamanca (HUS\/IBSAL) CIBERONC and Cancer Research Institute of Salamanca\u2010IBMCC (USAL\u2010CSIC) Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicia","family":"Ant\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Hematology University Hospital of Salamanca (HUS\/IBSAL) CIBERONC and Cancer Research Institute of Salamanca\u2010IBMCC (USAL\u2010CSIC) Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar","family":"Blanco","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospital of Salamanca (HUS\/IBSAL) Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis G.","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine University Hospital of Salamanca (HUS\/IBSAL), Salamanca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilar","family":"Tamayo","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine University Hospital of Salamanca (HUS\/IBSAL), Salamanca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica","family":"Gonz\u00e1lez\u2010Calle","sequence":"additional","affiliation":[{"name":"Department of Hematology University Hospital of Salamanca (HUS\/IBSAL) CIBERONC and Cancer Research Institute of Salamanca\u2010IBMCC (USAL\u2010CSIC) Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Jes\u00fas","family":"Vidal","sequence":"additional","affiliation":[{"name":"Department of Hematology University Hospital of Le\u00f3n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebeca","family":"Cuello","sequence":"additional","affiliation":[{"name":"Department of Hematology University Hospital of Valladolid, Valladolid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco Javier","family":"D\u00edaz G\u00e1lvez","sequence":"additional","affiliation":[{"name":"Department of Hematology University Hospital of Valladolid, Valladolid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Antonio","family":"Queiz\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Hematology Hospital of Segovia, Salamanca, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Mart\u00edn","sequence":"additional","affiliation":[{"name":"Department of Hematology University Hospital of Salamanca (HUS\/IBSAL) CIBERONC and Cancer Research Institute of Salamanca\u2010IBMCC (USAL\u2010CSIC) Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Department of Hematology University Hospital of Salamanca (HUS\/IBSAL) CIBERONC and Cancer Research Institute of Salamanca\u2010IBMCC (USAL\u2010CSIC) Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4120-2787","authenticated-orcid":false,"given":"Ram\u00f3n","family":"Garc\u00eda\u2010Sanz","sequence":"additional","affiliation":[{"name":"Department of Hematology University Hospital of Salamanca (HUS\/IBSAL) CIBERONC and Cancer Research Institute of Salamanca\u2010IBMCC (USAL\u2010CSIC) Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7335-3657","authenticated-orcid":false,"given":"M. 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