{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:43:25Z","timestamp":1787510605376,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,10,21]],"date-time":"2021-10-21T00:00:00Z","timestamp":1634774400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015570","name":"Blood Cancer UK","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100015570","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    While next\u2010generation sequencing technologies provide excellent strategies to screen for newly defined genetic abnormalities of prognostic or therapeutic significance in patients with B\u2010other\u2010acute lymphoblastic leukaemia (ALL), they are not widely available. We used a dual screening approach, incorporating fluorescence\n                    <jats:italic>in\u00a0situ<\/jats:italic>\n                    hybridisation (FISH) and Multiplex Ligation\u2010dependent Probe Amplification (MLPA), to establish the frequency and long\u2010term outcome of a representative cohort of specific subgroups of B\u2010other\u2010ALL recruited to the childhood ALL trial, UKALL2003. We focussed on abnormalities of known prognostic significance, including ABL\u2010class fusions and\n                    <jats:italic>ERG<\/jats:italic>\n                    deletions, as a surrogate marker for\n                    <jats:italic>DUX4<\/jats:italic>\n                    \u2010rearranged ALL. ABL\u2010class fusions accounted for ~4% of B\u2010other\u2010ALL and were associated with high levels of minimal residual disease (MRD; 14\/23 with MRD &gt;5%) and a high relapse rate (55\u00b77%) following treatment without tyrosine kinase inhibitor (TKI), confirming the importance of prospective screening with a view to incorporating TKI into therapy. Patients with deletions of\n                    <jats:italic>ERG<\/jats:italic>\n                    (~10% of B\u2010other\u2010ALL) had a 10\u2010year event\u2010free\u2010survival of 97\u00b72%, validating previous reports of their excellent outcome. Rearrangements of\n                    <jats:italic>ZNF384, MEF2D<\/jats:italic>\n                    and\n                    <jats:italic>NUTM1<\/jats:italic>\n                    were observed at low frequencies. Here, we estimate that approximately one third of B\u2010other\u2010ALL patients can be reliably classified into one of the known genetic subgroups using our dual screening method. This approach is rapid, accurate and readily incorporated into routine testing.\n                  <\/jats:p>","DOI":"10.1111\/bjh.17869","type":"journal-article","created":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T01:11:04Z","timestamp":1634865064000},"page":"753-763","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Genetic characterisation of childhood B\u2010other\u2010acute lymphoblastic leukaemia in UK patients by fluorescence\n                    <i>in\u00a0situ<\/i>\n                    hybridisation and Multiplex Ligation\u2010dependent Probe Amplification"],"prefix":"10.1111","volume":"196","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2030-1612","authenticated-orcid":false,"given":"Claire J.","family":"Schwab","sequence":"first","affiliation":[{"name":"Leukaemia Research Cytogenetics Group Translational and Clinical Research Institute Newcastle University Centre for Cancer Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Murdy","sequence":"additional","affiliation":[{"name":"Leukaemia Research Cytogenetics Group Translational and Clinical Research Institute Newcastle University Centre for Cancer Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellie","family":"Butler","sequence":"additional","affiliation":[{"name":"Leukaemia Research Cytogenetics Group Translational and Clinical Research Institute Newcastle University Centre for Cancer Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir","family":"Enshaei","sequence":"additional","affiliation":[{"name":"Leukaemia Research Cytogenetics Group Translational and Clinical Research Institute Newcastle University Centre for Cancer Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Winterman","sequence":"additional","affiliation":[{"name":"Leukaemia Research Cytogenetics Group Translational and Clinical Research Institute Newcastle University Centre for Cancer Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth E.","family":"Cranston","sequence":"additional","affiliation":[{"name":"Leukaemia Research Cytogenetics Group Translational and Clinical Research Institute Newcastle University Centre for Cancer Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarra","family":"Ryan","sequence":"additional","affiliation":[{"name":"Leukaemia Research Cytogenetics Group Translational and Clinical Research Institute Newcastle University Centre for Cancer Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilio","family":"Barretta","sequence":"additional","affiliation":[{"name":"Leukaemia Research Cytogenetics Group Translational and Clinical Research Institute Newcastle University Centre for Cancer Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe","family":"Hawking","sequence":"additional","affiliation":[{"name":"Leukaemia Research Cytogenetics Group Translational and Clinical Research Institute Newcastle University Centre for Cancer Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Murray","sequence":"additional","affiliation":[{"name":"Leukaemia Research Cytogenetics Group Translational and Clinical Research Institute Newcastle University Centre for Cancer Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace","family":"Antony","sequence":"additional","affiliation":[{"name":"Leukaemia Research Cytogenetics Group Translational and Clinical Research Institute Newcastle University Centre for Cancer Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajay","family":"Vora","sequence":"additional","affiliation":[{"name":"Department of Haematology Great Ormond Street Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony V.","family":"Moorman","sequence":"additional","affiliation":[{"name":"Leukaemia Research Cytogenetics Group Translational and Clinical Research Institute Newcastle University Centre for Cancer Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine J.","family":"Harrison","sequence":"additional","affiliation":[{"name":"Leukaemia Research Cytogenetics Group Translational and Clinical Research Institute Newcastle University Centre for Cancer Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70066-8"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/HS9.0000000000000053"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-018-0315-5"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1814397115"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11790"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3535"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3691"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/2643-3230.BCD-20-0229"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-12-758979"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1403088"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2012.06.005"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2014.55.4105"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.13492"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(08)70339-5"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-10-462358"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.111"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.151035"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.26116"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13331"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2013.324"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.66.5547"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2018.206961"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22439"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2013.095372"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.17093"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-09-670166"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2012.47.6770"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(14)70243-8"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20818"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019003191"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2010.08314.x"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2018025718"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-03-562918"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2017.74.3617"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2017.74.0449"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.69.6278"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-07-297135"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020008536"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(20)30353-7"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2013.277"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2013.282"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2017.186320"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1097\/HS9.0000000000000250"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-020-03953-3"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2019001008"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13115"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.17869","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bjh.17869","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.17869","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T18:41:43Z","timestamp":1692816103000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.17869"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,21]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1111\/bjh.17869"],"URL":"https:\/\/doi.org\/10.1111\/bjh.17869","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,21]]},"assertion":[{"value":"2021-05-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}