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A total of 942 bone marrow (BM) samples from 123 infants were studied for MFC\u2010MRD and FGT\u2010MRD. In total, 383\u00a0samples (40.7%) were concordantly MRD\u2010negative. MRD was detected by the two methods in 441 cases (46.8%); 99\u00a0samples (10.5%) were only FGT\u2010MRD\u2010positive and 19 (2.0%) were only MFC\u2010MRD\u2010positive. A final concordance rate of 87.4% was established. Most discordance occurred if residual leukaemia was present at levels close to the sensitivity limits. Neither the type of <jats:italic>KMT2A<\/jats:italic> fusion nor a new type of treatment hampering MFC methodology had an influence on the concordance rate. The prognostic value of MFC\u2010MRD and FGT\u2010MRD differed. MFC\u2010MRD was able to identify a rapid response at early time\u2010points, whereas FGT\u2010MRD was a reliable relapse predictor at later treatment stages. Additionally, the most precise risk definition was obtained when combining the two methods. Because of the high comparability in results, these two rather simple and inexpensive approaches could be good options of high clinical value.<\/jats:p>","DOI":"10.1111\/bjh.18021","type":"journal-article","created":{"date-parts":[[2021,12,31]],"date-time":"2021-12-31T05:19:53Z","timestamp":1640927993000},"page":"510-519","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Comparison of minimal residual disease measurement by multicolour flow cytometry and PCR for fusion gene transcripts in infants with acute lymphoblastic leukaemia with <i>KMT2A<\/i> gene rearrangements"],"prefix":"10.1111","volume":"201","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0889-6986","authenticated-orcid":false,"given":"Alexander","family":"Popov","sequence":"first","affiliation":[{"name":"National Research and Clinical Center for Pediatric Hematology, Oncology and Immunology  Moscow Russian 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