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However, studies have shown that patients with the e13a2 transcript were less likely to achieve major molecular response compared to those with e14a2 transcripts. Most quantitative polymerase chain reaction (PCR) assays for detection of the <jats:italic>BCR<\/jats:italic>\u2013<jats:italic>ABL1<\/jats:italic> fusion gene do not differentiate between the two transcripts and we therefore hypothesised that technical bias linked to the qPCR assay could partially explain the discrepancy in outcomes. We designed an e14a2\u2010specific assay and identified no difference in results compared to an e13a2\u00a0standard assay. We then demonstrated that the commercial e14a2\u00a0standards were causing a significant overestimation of the e13a2 transcripts. Finally, we reviewed patient management after the qPCR values were corrected, using our new evaluation. We concluded that despite statistically significant differences in qPCR results, there was no impact on patient management or outcome. We conclude that, at least in our institution, it would be inappropriate to perform separate assays for patients with e13a2 or e14a2.<\/jats:p>","DOI":"10.1111\/bjh.18026","type":"journal-article","created":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T12:27:54Z","timestamp":1641644874000},"page":"52-62","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Assessment of quantitative polymerase chain reaction for <i>BCR\u2013ABL1<\/i> transcripts in chronic myeloid leukaemia: Are improved outcomes in patients with e14a2 transcripts an artefact of\u00a0technology?"],"prefix":"10.1111","volume":"197","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4209-8096","authenticated-orcid":false,"given":"Katherine M.","family":"Dominy","sequence":"first","affiliation":[{"name":"Imperial Molecular Pathology Hammersmith Hospital Imperial College Healthcare NHS Trust  London 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