{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T12:37:28Z","timestamp":1774442248456,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T00:00:00Z","timestamp":1649721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Red blood cells (RBCs) lose plasma membrane in the spleen as they age, but the cells and molecules involved are yet to be identified. Sickle cell disease and infection by <jats:italic>Plasmodium falciparum<\/jats:italic> cause oxidative stress that induces aggregates of cross\u2010linked proteins with N\u2010linked high\u2010mannose glycans (HMGs). These glycans can be recognised by mannose\u2010binding lectins, including the mannose receptor (CD206), expressed on macrophages and specialised phagocytic endothelial cells in the spleen to mediate the extravascular haemolysis characteristic of these diseases. We postulated this system might also mediate removal of molecules and membrane in healthy individuals. Surface expression of HMGs on RBCs from patients who had previously undergone splenectomy was therefore assessed: high levels were indeed observable as large membrane aggregates. Glycomic analysis by mass spectrometry identified a mixture of Man<jats:sub>5\u20109<\/jats:sub>GlcNAc<jats:sub>2<\/jats:sub> structures. HMG levels correlated well with manual pit counts (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.75\u20130.85). To assess further whether HMGs might act as a splenic reticuloendothelial function test, we measured levels on RBCs from patients with potential functional hyposplenism, some of whom exhibited high levels that may indicate risk of complications.<\/jats:p>","DOI":"10.1111\/bjh.18164","type":"journal-article","created":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T12:07:48Z","timestamp":1649765268000},"page":"155-164","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Measurement of erythrocyte membrane mannoses to assess splenic function"],"prefix":"10.1111","volume":"198","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0122-5856","authenticated-orcid":false,"given":"Huan","family":"Cao","sequence":"first","affiliation":[{"name":"School of Medicine, Medical Sciences and Nutrition University of Aberdeen  Aberdeen UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abhinav","family":"Mathur","sequence":"additional","affiliation":[{"name":"Department of Haematology Aberdeen Royal Infirmary  Aberdeen UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5956-5191","authenticated-orcid":false,"given":"Charlotte","family":"Robertson","sequence":"additional","affiliation":[{"name":"Department of Haematology Aberdeen Royal Infirmary  Aberdeen UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aristotelis","family":"Antonopoulos","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Imperial College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadie","family":"Henderson","sequence":"additional","affiliation":[{"name":"School of Medicine, Medical Sciences and Nutrition University of Aberdeen  Aberdeen UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis\u2010Pierre","family":"Girard","sequence":"additional","affiliation":[{"name":"Department of Haematology Aberdeen Royal Infirmary  Aberdeen UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin Hien","family":"Wong","sequence":"additional","affiliation":[{"name":"School of Medicine, Medical Sciences and Nutrition University of Aberdeen  Aberdeen UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam","family":"Davie","sequence":"additional","affiliation":[{"name":"School of Medicine, Medical Sciences and Nutrition University of Aberdeen  Aberdeen UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonja","family":"Wright","sequence":"additional","affiliation":[{"name":"Department of Haematology Aberdeen Royal Infirmary  Aberdeen UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6129-4300","authenticated-orcid":false,"given":"John","family":"Brewin","sequence":"additional","affiliation":[{"name":"Department of Haematology King's College Hospital  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3647-1050","authenticated-orcid":false,"given":"David C.","family":"Rees","sequence":"additional","affiliation":[{"name":"Department of Haematology King's College Hospital  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Dell","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Imperial College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stuart M.","family":"Haslam","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Imperial College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Vickers","sequence":"additional","affiliation":[{"name":"School of Medicine, Medical Sciences and Nutrition University of Aberdeen  Aberdeen UK"},{"name":"Department of Haematology Aberdeen Royal Infirmary  Aberdeen UK"},{"name":"Scottish National Blood Transfusion Service  Aberdeen UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,4,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V14.4.399.399"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1669"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(98)80024-2"},{"issue":"2","key":"e_1_2_7_5_1","first-page":"107","article-title":"Innate immune and non\u2010immune mediators of erythrocyte clearance","volume":"50","author":"Lutz HU","year":"2004","journal-title":"Cell Mol Biol (Noisy\u2010le\u2010Grand)"},{"issue":"4","key":"e_1_2_7_6_1","first-page":"387","article-title":"Mechanisms tagging senescent red blood cells for clearance in healthy humans","volume":"25","author":"Lutz HU","year":"2013","journal-title":"Front Physiol"},{"issue":"8","key":"e_1_2_7_7_1","first-page":"73","article-title":"From the cradle to the grave: the role of macrophages in erythropoiesis and erythrophagocytosis","volume":"8","author":"Klei TR","year":"2017","journal-title":"Front Immunol"},{"issue":"5","key":"e_1_2_7_8_1","first-page":"9","article-title":"Of macrophages and red blood cells; a complex love story","volume":"30","author":"Back DZ","year":"2014","journal-title":"Front Physiol"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2017013094"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1606751113"},{"issue":"14","key":"e_1_2_7_11_1","first-page":"1569","article-title":"Turning over a new leaf on turning over RBCs","volume":"136","author":"Zimring JC","year":"2020","journal-title":"Blood"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.655393"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830420110"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.01607"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1806501115"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-019-0129-4"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-71096-6"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21814-z"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MCHD\u20100036"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1992.tb04604.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(06)15005-3"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr7008252"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)35413-4"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwz045"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24421"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000517338"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2015.160"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.57.5.338"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-5080fje"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2013.12.008"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-397863-9.00006-7"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2012.05.006"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20160005"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-474-2_32"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2011.11.007"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan0218"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-04-637702"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0512231"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700327"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwj077"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10020296"},{"issue":"5","key":"e_1_2_7_42_1","first-page":"562","article-title":"Erythrocytes as a preferential target of oxidative stress in blood","volume":"55","author":"Fujii J","year":"2021","journal-title":"Free Radic Res"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-017-0392-4"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep35234"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M207057200"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.l4183"},{"issue":"6","key":"e_1_2_7_47_1","first-page":"607","article-title":"Recommended screening and preventive practices for long\u2010term survivors after hematopoietic cell transplantation","volume":"55","author":"Majhail NS","year":"2014","journal-title":"Rinsho Ketsueki"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.18164","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bjh.18164","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.18164","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T06:28:57Z","timestamp":1693463337000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.18164"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,12]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1111\/bjh.18164"],"URL":"https:\/\/doi.org\/10.1111\/bjh.18164","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,12]]},"assertion":[{"value":"2021-10-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}