{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:39:37Z","timestamp":1790746777211,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,10,20]],"date-time":"2022-10-20T00:00:00Z","timestamp":1666224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We evaluated CD34<jats:sup>+<\/jats:sup> cells in a single\u2010centre series of 49 consecutive patients with myelofibrosis (MF) at baseline and during ruxolitinib therapy and examined any association with spleen response. The median (range) absolute number of circulating CD34<jats:sup>+<\/jats:sup> cells was 0.0835\u00a0(0.001\u20131.528)\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>\/L at diagnosis, and 0.123\u00a0(0.002\u20131.528)\u2009\u00d7\u200910<jats:sup>9<\/jats:sup>\/L at ruxolitinib start. With the exception of a transient increase after 3\u2009months of ruxolitinib therapy, a progressive reduction in CD34<jats:sup>+<\/jats:sup> cells count was documented, down to a minimum of 0.063\u00a0\u00d7\u200910<jats:sup>9<\/jats:sup>\/L after 36\u2009months. We then assessed the association between spleen diameter expressed as the distance from the left costal margin (outcome) and log(CD34<jats:sup>+<\/jats:sup>) cells count using random\u2010intercept and random slope multivariable regression models to take into account within subject correlation: after adjusting for time and ruxolitinib dosage, we estimated a 0.7\u2009cm increase (95% confidence interval 0.2\u20131.2, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.003) in spleen length for each unit increase in log(CD34<jats:sup>+<\/jats:sup>) cells count (\u00d7\u200910<jats:sup>9<\/jats:sup>\/L). Although our study has some limitations, mainly related to its retrospective design, our approach may introduce a reproducible and simple tool that could facilitate the assessment of spleen response more objectively in patients with MF treated with ruxolitinib.<\/jats:p>","DOI":"10.1111\/bjh.18526","type":"journal-article","created":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T04:49:41Z","timestamp":1666327781000},"page":"315-322","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Trend of circulating CD34<sup>+<\/sup> cells in patients with myelofibrosis: Association with spleen response during ruxolitinib treatment"],"prefix":"10.1111","volume":"200","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4401-0812","authenticated-orcid":false,"given":"Alessandra","family":"Iurlo","sequence":"first","affiliation":[{"name":"Hematology Division, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico  Milan 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