{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T06:36:02Z","timestamp":1767162962718,"version":"build-2238731810"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T00:00:00Z","timestamp":1677715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>\n                    <jats:styled-content>Summary<\/jats:styled-content>\n                  <\/jats:title>\n                  <jats:p>\n                    Clinical manifestations and laboratory parameters of haemostasis were investigated in 23 children with newly diagnosed immune thrombocytopenia (ITP) before and after intravenous immunoglobulin (IVIg) treatment. ITP patients with platelet counts of less than 20\u2009\u00d7\u200910\n                    <jats:sup>9<\/jats:sup>\n                    \/L and mild bleeding symptoms, graded by a standardized bleeding score (BS), were compared with healthy children with normal platelet counts and children with chemotherapy\u2010related thrombocytopenia. Markers of platelet activation and platelet apoptosis in the absence and presence of platelet activators were analysed by flow cytometry; thrombin generation in plasma was determined. ITP patients at diagnosis presented with increased proportions of platelets expressing CD62P and CD63 and activated caspases, and with decreased thrombin generation. Thrombin\u2010induced activation of platelets was reduced in ITP compared with controls, while increased proportions of platelets with activated caspases were observed. Children with a higher BS had lower proportions of CD62P\u2010expressing platelets compared with those with a lower BS. IVIg treatment increased the number of reticulated platelets, the platelet count to more than 20\u2009\u00d7\u200910\n                    <jats:sup>9<\/jats:sup>\n                    \/L and improved bleeding in all patients. Decreased thrombin\u2010induced platelet activation, as well as thrombin generation, were ameliorated. Our results indicate that IVIg treatment helps to counteract diminished platelet function and coagulation in children with newly diagnosed ITP.\n                  <\/jats:p>","DOI":"10.1111\/bjh.18702","type":"journal-article","created":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T04:50:56Z","timestamp":1677732656000},"page":"1209-1219","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<scp>IVI<\/scp>\n                    g treatment increases thrombin activation of platelets and thrombin generation in paediatric patients with immune thrombocytopenia"],"prefix":"10.1111","volume":"201","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4745-8572","authenticated-orcid":false,"given":"Markus","family":"Schmugge","sequence":"first","affiliation":[{"name":"Division of Hematology, Children's Research Center University Children's Hospital Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1939-376X","authenticated-orcid":false,"given":"Francesca Daniela","family":"Franzoso","sequence":"additional","affiliation":[{"name":"Division of Hematology, Children's Research Center University Children's Hospital Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeannine","family":"Winkler","sequence":"additional","affiliation":[{"name":"Division of Hematology, Children's Research Center University Children's Hospital Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Kroiss","sequence":"additional","affiliation":[{"name":"Division of Hematology Oncology, Children's Research Center University Children's Hospital Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Seiler","sequence":"additional","affiliation":[{"name":"Division of Hematology Emergency Department, Children's Research Center University Children's Hospital Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Speer","sequence":"additional","affiliation":[{"name":"Center for Laboratory Medicine Center for Laboratory Medicine  St. Gallen Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret L.","family":"Rand","sequence":"additional","affiliation":[{"name":"Division of Haematology\/Oncology, Translational Medicine, Research Institute The Hospital for Sick Children  Toronto Ontario Canada"},{"name":"Departments of Laboratory Medicine &amp; Pathobiology, Biochemistry, and Paediatrics University of Toronto  Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-138487"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2011.04506.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcl.2008.01.009"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-06-225565"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2019001343"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-02-830844"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.291.5503.484"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1997.2423059.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-05-1475"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1538-7836.2003.00091.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm6020016"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0000000000000612"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm921"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-148288"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8737"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020008238"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.13.4254.h8004254_4254_4260"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1615653"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-07-515536"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1991.tb00529.x"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0049-3848(02)00260-8"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2013.087874"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2007.01855.x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-02-628461"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2020.00015"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-01-0275"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.08973.x"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1808217115"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000031340.68494.34"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1613868"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2017.37.6.484"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.14412"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1067\/mpd.2002.128547"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)04143-3"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1203\/01.PDR.0000081294.26060.4B"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH12-06-0404"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2010.03766.x"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-11-321398"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2006.05981.x"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biologicals.2014.04.002"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12280"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(05)80400-7"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012369367-9\/50792-8"}],"updated-by":[{"DOI":"10.1111\/bjh.19402","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T00:00:00Z","timestamp":1712188800000}}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.18702","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bjh.18702","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bjh.18702","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T02:12:59Z","timestamp":1692411179000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bjh.18702"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,2]]},"references-count":43,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1111\/bjh.18702"],"URL":"https:\/\/doi.org\/10.1111\/bjh.18702","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,2]]},"assertion":[{"value":"2022-06-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}