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The detailed pathogenesis of IE remains unclear. In this study, we used single\u2010cell RNA sequencing (scRNA\u2010seq) to examine IE in Th3\/+ \u03b2\u2010thalassaemic mice. The results showed that the erythroid group was remarkably expanded, and genes involved in biological processes such as iron metabolism, haeme synthesis, protein folding, and response to heat were significantly upregulated from erythroid progenitors to reticulocytes in \u03b2\u2010thalassaemic mice. In particular, we identified a unique cell population close to reticulocytes, named ThReticulocytes, characterised by a high level of heat shock protein 70 (Hsp70) expression and dysregulation of iron metabolism and haeme synthesis signalling. Treatment of \u03b2\u2010thalassaemic mice with the haeme oxygenase inhibitor tin\u2010mesoporphyrin effectively improved the iron disorder and IE, and the ThReticulocyte population and Hsp70 expression were significantly suppressed. 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