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To investigate whether alterations in the BM niche cells are associated with PC, we analysed CD271<jats:sup>+<\/jats:sup> stromal cells in BM biopsy specimens and the cytokine profiles of the BM and serum obtained before and on day 28 after CAR T\u2010cell infusion. Imaging analyses of the BM biopsy specimens revealed that CD271<jats:sup>+<\/jats:sup> niche cells were severely impaired after CAR T\u2010cell infusion in patients with PC. Cytokine analyses after CAR T\u2010cell infusion showed that CXC chemokine ligand 12 and stem cell factor, niche factors essential for haematopoietic recovery, were significantly decreased in the BM of patients with PC, suggesting reduced niche cell function. The levels of inflammation\u2010related cytokines on day 28 after CAR T\u2010cell infusion were consistently high in the BM of patients with PC. Thus, we demonstrate for the first time that BM niche disruption and sustained elevation of inflammation\u2010related cytokines in the BM following CAR T\u2010cell infusion are associated with subsequent PC.<\/jats:p>","DOI":"10.1111\/bjh.18747","type":"journal-article","created":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T06:59:09Z","timestamp":1678345149000},"page":"294-307","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Bone marrow microenvironment disruption and sustained inflammation with prolonged haematologic toxicity after <scp>CAR<\/scp> T\u2010cell therapy"],"prefix":"10.1111","volume":"202","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8021-4732","authenticated-orcid":false,"given":"Wataru","family":"Kitamura","sequence":"first","affiliation":[{"name":"Department of Hematology and Oncology Okayama University Hospital  Okayama Japan"},{"name":"Department of Hematology Oncology and 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